A secondary wave of neutrophil infiltration causes necrosis and ulceration in lesions of experimental American cutaneous leishmaniasis
| Autor: | Alex G. Peniche, Gloria I. Palma, E. Yaneth Osorio, Bruno L. Travi, Diana L. Bonilla, Peter C. Melby |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology Necrosis Neutrophils lcsh:Medicine Cathepsin G Pathology and Laboratory Medicine Biochemistry Parasite Load chemistry.chemical_compound White Blood Cells 0302 clinical medicine Animal Cells Cricetinae Medicine and Health Sciences Medicine lcsh:Science Immune Response Leishmania Mammals Multidisciplinary biology Elastase Proteases Enzymes Neutrophil Infiltration Myeloperoxidase Vertebrates Hamsters Female medicine.symptom Cellular Types Infiltration (medical) Research Article medicine.medical_specialty Immune Cells Immunology Leishmaniasis Cutaneous Inflammation Nitric Oxide Rodents Proinflammatory cytokine Lesion 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Parasitic Diseases Animals Humans Blood Cells business.industry Macrophages lcsh:R Organisms Biology and Life Sciences Proteins Cell Biology medicine.disease United States Disease Models Animal 030104 developmental biology chemistry Amniotes biology.protein Lesions Enzymology lcsh:Q business Reactive Oxygen Species 030215 immunology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 6, p e0179084 (2017) |
| ISSN: | 1932-6203 |
| Popis: | We evaluated the importance of neutrophils in the development of chronic lesions caused by L. Viannia spp. using the hamster as experimental model of American Cutaneous Leishmaniasis (ACL). Neutrophils infiltrated the lesion within the first six hours post-infection. Inhibition of this early infiltration using a polyclonal antibody or cyclophosphamide was associated with transient parasite control but the protective effect vanished when lesions became clinically apparent. At lesion onset (approximately 10 days p.i.), there was an increased proportion of both uninfected and infected macrophages, and subsequently a second wave of neutrophils infiltrated the lesion (after 19 days p.i.) This second neutrophil infiltration was associated with lesion necrosis and ulceration (R2 = 0.75) and maximum parasite burden. Intradermal delivery of N-formylmethionyl-leucyl-phenylalanine (fMLP), aimed to increase neutrophil infiltration, resulted in larger lesions with marked necrosis and higher parasite burden than in mock treated groups (p |
| Databáze: | OpenAIRE |
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