Contribution of classical complement activation and IgM to the control of Rickettsia infection
| Autor: | Jack H. Cook, Sean P. Riley, Jinyi C. Zhu, Mustapha Dahmani |
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| Rok vydání: | 2021 |
| Předmět: |
Complement C1q
Intracellular parasite Rickettsia Infections Biology bacterial infections and mycoses biology.organism_classification Antibodies Bacterial Microbiology In vitro Complement (complexity) Complement system Mice Inbred C57BL Mice Classical complement pathway Immune system Rickettsia Immunoglobulin M Immunity Immunology Animals Humans bacteria Complement Pathway Classical Molecular Biology |
| Zdroj: | Molecular Microbiology. 116:1476-1488 |
| ISSN: | 1365-2958 0950-382X |
| Popis: | Pathogenic Rickettsia are obligate intracellular bacteria and the etiologic agents of many life-threatening infectious diseases. Due to the serious nature of these infections, it is imperative to both identify the responsive immune sensory pathways and understand the associated immune mechanisms that restrict Rickettsia proliferation. Previous studies have demonstrated that the mammalian complement system is both activated during Rickettsia infection and contributes to the immune response to infection. To further define this component of the mammalian anti-Rickettsia immune response, we sought to identify the mechanism(s) of complement activation during Rickettsia infection. We have employed a series of in vitro and in vivo models of infection to investigate the role of the classical complement activation pathway during Rickettsia infection. Depletion or elimination of complement activity demonstrates that both C1q and pre-existing IgM contribute to complement activation; thus implicating the classical complement system in Rickettsia-mediated complement activation. Elimination of the classical complement pathway from mice increases susceptibility to R. australis infection with both increased bacterial loads in multiple tissues and decreased immune activation markers. This study highlights the role of the classical complement pathway in immunity against Rickettsia and implicates resident Rickettsia-responsive IgM in the response to infection. |
| Databáze: | OpenAIRE |
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