Burn Trauma Acutely Increases the Respiratory Capacity and Function of Liver Mitochondria
| Autor: | Fredrick J. Bohanon, Nisha Bhattarai, Tracy Toliver-Kinsky, Anesh Prasai, Ravi S. Radhakrishnan, Xiaofu Wang, Amina El Ayadi, Jayson W. Jay, Yesenia Rojas-Khalil, Craig Porter, David N. Herndon, Celeste C. Finnerty, Omar Nunez Lopez |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Mitochondria Liver Citrate (si)-Synthase Mitochondrion Critical Care and Intensive Care Medicine Article Electron Transport Rats Sprague-Dawley Mice 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine Respiration Animals Humans Medicine Citrate synthase Intensive care medicine Mice Inbred BALB C biology medicine.diagnostic_test business.industry 030208 emergency & critical care medicine Hep G2 Cells Metabolism Mitochondria Rats 030104 developmental biology Endocrinology Mitochondrial biogenesis Coenzyme Q – cytochrome c reductase Hepatocytes Emergency Medicine Hypermetabolism biology.protein Burns business |
| Zdroj: | Shock. 49:466-473 |
| ISSN: | 1540-0514 1073-2322 |
| Popis: | BACKGROUND A complete understanding of the role of the liver in burn-induced hypermetabolism is lacking. We investigated the acute effect of severe burn trauma on liver mitochondrial respiratory capacity and coupling control as well as the signaling events underlying these alterations. METHODS Male BALB/c mice (8-12 weeks) received full-thickness scald burns on ∼30% of the body surface. Liver tissue was harvested 24 h postinjury. Mitochondrial respiration was determined by high-resolution respirometry. Citrate synthase activity was determined as a proxy of mitochondrial density. Male Sprague-Dawley rats received full-thickness scald burns to ∼60% of the body surface. Serum was collected 24 h postinjury. HepG2 cells were cultured with serum-enriched media from either sham- or burn-treated rats. Protein levels were analyzed via western blot. RESULTS Mass-specific (P = 0.01) and mitochondrial-specific (P = 0.01) respiration coupled to ATP production significantly increased in the liver after burn. The respiratory control ratio for ADP (P = 0.04) and the mitochondrial flux control ratio (P = 0.03) were elevated in the liver of burned animals. Complex III and Complex IV protein abundance in the liver increased after burn by 17% and 14%, respectively. Exposure of HepG2 cells to serum from burned rats increased the pAMPKα:AMPKα ratio (P |
| Databáze: | OpenAIRE |
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