Complex formation with protamine prolongs the thrombin-inhibiting effect of DNA aptamer in vivo

Autor: T.A. Shishkina, V.A. Spiridonova, E.V. Golubkina, O.S. Dyukareva, D.M. Nikulina, T.M. Novikova, N.N. Trizno
Rok vydání: 2018
Předmět:
Zdroj: Biochimie. 145:158-162
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2017.09.010
Popis: Antithrombin DNA aptamersRE31 are single-chain oligonucleotides that fold into three-dimensional forms allowing them to bind the enzyme with high affinity and inhibit its activity in vivo. They are rapidly degraded by a nonspecific nuclease, and, to prolong the lifetime of the aptamer DNA in the bloodstream, it is necessary to coat it with a polymer envelope. A new approach to solving this problem based on preparation of DNA–polyelectrolyte complexes with a minimal particle size that can circulate with blood flow. In our experiments, the negatively charged aptamer DNA RE31 was coated step-by-step with positively charged protamine. They had protamine/aptamer ratios of 0.2/1 and 0.4/1 by charge, with particle size being determined by dynamic light scattering. The aptamer DNA–protamine complexes were administered to rats, followed by ex vivo analysis of blood samples. The results showed that prothrombin time (PT) increased by a factor of 5.6–6.7 within 2 h after injection and remained at approximately the same level for 6 h, while injections of pure protamine did not lead to any noticeable change in clotting time. Thus, complexation with protamine proved to prolong the inhibitory activity of the RE31 DNA aptamer.
Databáze: OpenAIRE
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