Isolation, identification and characterization of apigenin from Justicia gendarussa and its anti-inflammatory activity
| Autor: | Antony Helen, V. Sabu, G. Sindhu, Arun A. Rauf, Kavitha S. Kumar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.drug_class Immunology Flavonoid Anti-Inflammatory Agents Pharmacology Carrageenan Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Justicia medicine Immunology and Allergy Animals Edema Humans Apigenin Rats Wistar Cells Cultured chemistry.chemical_classification biology Chemistry NF-kappa B biology.organism_classification Justicia gendarussa Bioactive compound In vitro Lipoproteins LDL Toll-Like Receptor 4 030104 developmental biology Prostaglandin-Endoperoxide Synthases 030220 oncology & carcinogenesis Leukocytes Mononuclear Bioflavonoid Cytokines |
| Zdroj: | International immunopharmacology. 59 |
| ISSN: | 1878-1705 |
| Popis: | Inflammatory responses during chronic diseases such as atherosclerosis, cancer etc., are harmful to host organisms. Generally NSAIDs are used to treat against these severe conditions but due to its adverse effects studies are going on with medicinal plants, since they are rich in bioactive compounds. Justicia gendarussa is one such plant which has been used as a remedial measure for treating inflammatory diseases since ancient time. Thus the present study involved in the isolation, characterization and identification of apigenin (flavonoid) from this plant and to elucidate its molecular mechanism against inflammation via TLR-NF-κB signaling pathway using ox-LDL induced hPBMCs in in vitro model. Methanolic extract was used for the isolation process and results showed that the F6 fraction collected from ethyl acetate through column chromatography showed 89% paw edema inhibition at a dose of 10 mg/kg in carrageenan induced rats. Purification of F6 by TLC with toluene: chloroform: acetone (8:5:7) and further characterization by 1HNMR indicated the presence of bioactive compound, apigenin. In vitro studies revealed that pretreatment of ox-LDL induced hPBMCs with apigenin (25 μM) significantly (P |
| Databáze: | OpenAIRE |
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