Ultrastructural Profile Combined with Immunohistochemistry of a Hepatic Progenitor Cell Line in Pediatric Autoimmune Hepatitis: New Insights into the Morphological Pattern of the Disease
Autor: | Piotr Sobaniec, Maria Elzbieta Sobaniec-Lotowska, Joanna Maria Lotowska |
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Rok vydání: | 2021 |
Předmět: |
Male
Pathology transitional hepatic stellate cells (T-HSCs) Autoimmune hepatitis 0302 clinical medicine immune system diseases transmission electron microscopy (TEM) hyperactive Kupffer cells Biology (General) Child education.field_of_study liver fibrogenesis Stem Cells Liver cell Age Factors Cell Differentiation General Medicine Immunohistochemistry Hepatitis Autoimmune medicine.anatomical_structure Liver Child Preschool 030220 oncology & carcinogenesis Hepatocyte Female 030211 gastroenterology & hepatology medicine.medical_specialty Adolescent QH301-705.5 Kupffer Cells Population pediatric autoimmune hepatitis (AIH) Article Cell Line 03 medical and health sciences Cytokeratin Microscopy Electron Transmission Predictive Value of Tests medicine Humans immunohistochemistry for CK7 hepatic progenitor cell line (HPC line) Progenitor cell education Cell Proliferation Retrospective Studies ultrastructural types of HPCs business.industry Keratin-7 medicine.disease digestive system diseases Liver Regeneration Hepatocytes Hepatic stellate cell business Biomarkers |
Zdroj: | Cells Volume 10 Issue 8 Cells, Vol 10, Iss 1899, p 1899 (2021) |
ISSN: | 2073-4409 |
DOI: | 10.3390/cells10081899 |
Popis: | Considering that the heterogenic population of a hepatic progenitor cell line (HPCL) can play a vital role in autoimmune hepatitis (AIH), we decided to conduct pioneering retrospective evaluation of these cells in pediatric AIH by means of transmission electron microscopy (TEM). The aim of the study was to assess the ultrastructure of the HPCL in children with untreated AIH. Ultrastructural analysis of the HPCL population, preceded by immunohistochemical staining for cytokeratin 7 (CK7), was performed using pretreatment liver biopsies from 23 children with clinicopathologically diagnosed AIH. Immunohistochemical assessment for CK7 allowed detection of proliferating immature epithelial cells differentiating towards periportal and intralobular intermediate hepatocytes without marked formation of ductular reactions in AIH children. Using TEM, we distinguished three morphological types of HPCs: I—the most undifferentiated progenitor cells III—intermediate hepatocyte-like cells II—intermediate bile duct cells. Most frequent were the cells differentiating towards hepatocytes, most rare—those differentiating towards cholangiocytes. The results indicate that an HPCL may be an important source of hepatocyte regeneration. Ultrastructural analyses of the HPCL population, combined with immunohistochemistry for CK7, might be a useful tool to evaluate liver cell regeneration, including fibrogenesis, and may help better understand the morphological pattern of the disease, in pediatric AIH. Frequent appearance of an HPCL in the vicinity of fibrotic foci, often accompanied by hyperactive Kupffer cells and transitional hepatic stellate cells, may indicate their significant involvement in liver fibrogenesis. |
Databáze: | OpenAIRE |
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