Ultrastructural Profile Combined with Immunohistochemistry of a Hepatic Progenitor Cell Line in Pediatric Autoimmune Hepatitis: New Insights into the Morphological Pattern of the Disease

Autor: Piotr Sobaniec, Maria Elzbieta Sobaniec-Lotowska, Joanna Maria Lotowska
Rok vydání: 2021
Předmět:
Male
Pathology
transitional hepatic stellate cells (T-HSCs)
Autoimmune hepatitis
0302 clinical medicine
immune system diseases
transmission electron microscopy (TEM)
hyperactive Kupffer cells
Biology (General)
Child
education.field_of_study
liver fibrogenesis
Stem Cells
Liver cell
Age Factors
Cell Differentiation
General Medicine
Immunohistochemistry
Hepatitis
Autoimmune

medicine.anatomical_structure
Liver
Child
Preschool

030220 oncology & carcinogenesis
Hepatocyte
Female
030211 gastroenterology & hepatology
medicine.medical_specialty
Adolescent
QH301-705.5
Kupffer Cells
Population
pediatric autoimmune hepatitis (AIH)
Article
Cell Line
03 medical and health sciences
Cytokeratin
Microscopy
Electron
Transmission

Predictive Value of Tests
medicine
Humans
immunohistochemistry for CK7
hepatic progenitor cell line (HPC line)
Progenitor cell
education
Cell Proliferation
Retrospective Studies
ultrastructural types of HPCs
business.industry
Keratin-7
medicine.disease
digestive system diseases
Liver Regeneration
Hepatocytes
Hepatic stellate cell
business
Biomarkers
Zdroj: Cells
Volume 10
Issue 8
Cells, Vol 10, Iss 1899, p 1899 (2021)
ISSN: 2073-4409
DOI: 10.3390/cells10081899
Popis: Considering that the heterogenic population of a hepatic progenitor cell line (HPCL) can play a vital role in autoimmune hepatitis (AIH), we decided to conduct pioneering retrospective evaluation of these cells in pediatric AIH by means of transmission electron microscopy (TEM). The aim of the study was to assess the ultrastructure of the HPCL in children with untreated AIH. Ultrastructural analysis of the HPCL population, preceded by immunohistochemical staining for cytokeratin 7 (CK7), was performed using pretreatment liver biopsies from 23 children with clinicopathologically diagnosed AIH. Immunohistochemical assessment for CK7 allowed detection of proliferating immature epithelial cells differentiating towards periportal and intralobular intermediate hepatocytes without marked formation of ductular reactions in AIH children. Using TEM, we distinguished three morphological types of HPCs: I—the most undifferentiated progenitor cells
III—intermediate hepatocyte-like cells
II—intermediate bile duct cells. Most frequent were the cells differentiating towards hepatocytes, most rare—those differentiating towards cholangiocytes. The results indicate that an HPCL may be an important source of hepatocyte regeneration. Ultrastructural analyses of the HPCL population, combined with immunohistochemistry for CK7, might be a useful tool to evaluate liver cell regeneration, including fibrogenesis, and may help better understand the morphological pattern of the disease, in pediatric AIH. Frequent appearance of an HPCL in the vicinity of fibrotic foci, often accompanied by hyperactive Kupffer cells and transitional hepatic stellate cells, may indicate their significant involvement in liver fibrogenesis.
Databáze: OpenAIRE
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