Selective in vitro reversal of the insulin resistance of glucose transport in denervated rat skeletal muscle
| Autor: | Maria G. Buse, S. L. Dutton, M. O. Sowell |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Time Factors Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Muscle Proteins Insulin resistance Physiology (medical) Internal medicine medicine Animals Insulin Amino Acids Glycogen synthase Glucocorticoids Denervation biology Muscles Glucose transporter Biological Transport Rats Inbred Strains medicine.disease Muscle Denervation Rats Insulin oscillation Insulin receptor Glucose Endocrinology Glycogenesis biology.protein Insulin Resistance |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 257:E418-E425 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1989.257.3.e418 |
| Popis: | Denervation (24 h) of skeletal muscle causes severe postreceptor insulin resistance of glucose transport and glycogen synthesis that is demonstrable in isolated muscles after short (30 min) preincubations. After longer preincubations (2-4 h), the insulin response of glucose transport increased to normal, whereas glycogen synthesis remained insulin resistant. Basal and insulin-stimulated amino acid transport were significantly lower in denervated muscles than in controls after short or long incubations, although the percentage stimulation of transport by insulin was not significantly different. The development of glucose transport insulin resistance after denervation was not attributable to increased sensitivity to glucocorticoids or adenosine. The selective in vitro reversal of glucose transport insulin resistance was not dependent on medium composition, did not require protein or prostaglandin synthesis, and could not be attributed to release of a positive regulator into the medium. The data suggest 1) the insulin receptor in muscle stimulates glucose transport by a signaling pathway that is not shared by other insulin-sensitive effector systems, and 2) denervation may affect insulin receptor signal transduction at more than one site. |
| Databáze: | OpenAIRE |
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