Morphine modulates monocyte–macrophage conversion phase
| Autor: | Amit Dinda, Naoko Hitosugi, Pravin C. Singhal, Ikuske Hatsukari |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Narcotic Antagonists
Immunology Monocytes Superoxide dismutase chemistry.chemical_compound Cell Movement Superoxides parasitic diseases Cell Adhesion medicine Humans Macrophage Receptor Cells Cultured Morphine biology Superoxide Macrophages Monocyte Cell Differentiation Cell biology medicine.anatomical_structure chemistry Receptors Opioid biology.protein Phorbol Tetradecanoylphorbol Acetate Opiate medicine.drug |
| Zdroj: | Cellular Immunology. 239:41-48 |
| ISSN: | 0008-8749 |
| Popis: | Monocyte migration and their activation into the macrophage phenotype play a role in the modulation of tissue injury. We studied the effect of morphine on the monocyte-macrophage conversion phase (MMCP). Phorbol 12-myristate 13-acetate (PMA) activated THP-1 cells and promoted their adhesion to the substrate. Morphine inhibited PMA-induced MMCP. However, opiate receptor antagonists attenuated this effect of morphine. Interestingly, PMA as well as morphine-stimulated superoxide production by monocytes. Superoxide dismutase (SOD) not only inhibited PMA-mediated MMCP but also attenuated the inhibitory effect of morphine. PMA not only enhanced adhesion of monocytes to a filter but also promoted their migration. These findings suggest that the PMA-induced macrophage phenotype conversion may be accelerating their migration; whereas, morphine may be preventing the migration of monocytes by inhibiting MMCP. |
| Databáze: | OpenAIRE |
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