Saccharomyces boulardii Improves Intestinal Epithelial Cell Restitution by Inhibiting αvβ5 Integrin Activation State

Autor: Carole Siret, Jacques Marvaldi, Véronique Rigot, Sonia Bastonero, Frédéric André, Dorota Czerucka, Chloé Terciolo, Emilie Pellegrino, Alexandra Canonici, Dominique Lombardo
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Integrins
lcsh:Medicine
Inflammatory bowel disease
Collagen receptor
Mice
Crohn Disease
Cell Movement
Molecular Cell Biology
Morphogenesis
Signaling in Cellular Processes
lcsh:Science
Multidisciplinary
Intestinal epithelium
Extracellular Matrix
Protein Transport
Medicine
Vitronectin
Female
Cellular Types
Saccharomyces boulardii
Research Article
Signal Transduction
Protein Binding
Integrin
Cell Migration
Gastroenterology and Hepatology
Biology
Focal adhesion
Saccharomyces
Cell Line
Tumor
medicine
Cell Adhesion
Ulcerative Colitis
Animals
Humans
Receptors
Vitronectin
Cell adhesion
Extracellular Matrix Adhesions
lcsh:R
Inflammatory Bowel Disease
Epithelial Cells
Feeding Behavior
medicine.disease
biology.organism_classification
Mice
Inbred C57BL
Molecular Weight
Enterocytes
Immunology
Cancer research
biology.protein
lcsh:Q
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 9, p e45047 (2012)
ISSN: 1932-6203
Popis: Intestinal epithelial cell damage is frequently seen in the mucosal lesions of infectious or inflammatory bowel diseases such as ulcerative colitis or Crohn's disease. Complete remission of these diseases requires both the disappearance of inflammation and the repair of damaged epithelium. Saccharomyces boulardii (Sb, Biocodex) is a non-pathogenic yeast widely used as a preventive and therapeutic probiotic for the prevention and treatment of diarrhea and other gastrointestinal disorders. We recently showed that it enhances the repair of intestinal epithelium through activation of α2β1 integrin collagen receptors. In the present study, we demonstrated that α2β1 integrin is not the sole cell-extracellular matrix receptor involved during Sb-mediated intestinal restitution. Indeed, by using cell adhesion assays, we showed that Sb supernatant contains heat sensitive molecule(s), with a molecular weight higher than 9 kDa, which decreased αvβ5 integrin-mediated adhesion to vitronectin by competing with the integrin. Moreover, Sb-mediated changes in cell adhesion to vitronectin resulted in a reduction of the αvβ5signaling pathway. We used a monolayer wounding assay that mimics in vivo cell restitution to demonstrate that down-modulation of the αvβ5 integrin-vitronectin interaction is related to Sb-induced cell migration. We therefore postulated that Sb supernatant contains motogenic factors that enhance cell restitution through multiple pathways, including the dynamic fine regulation of αvβ5 integrin binding activity. This could be of major importance in diseases characterized by severe mucosal injury, such as inflammatory and infectious bowel diseases.
Databáze: OpenAIRE
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