Penetration of an antimicrobial zinc-sugar alcohol complex into Streptococcus mutans biofilms
| Autor: | Jeong Rae Lee, Ahn Jae-Hyun, Jeong Yong-Beom, Sang-Min Lee, Jong Hyun Lim, Sang-Hun Song |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology chemistry.chemical_element lcsh:Medicine Zinc Erythritol Polysaccharide Article Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Extracellular polymeric substance Sugar Alcohols Anti-Infective Agents Coordination Complexes Polysaccharides Humans Sugar alcohol lcsh:Science chemistry.chemical_classification Multidisciplinary Microscopy Confocal biology lcsh:R Biofilm 030206 dentistry Antimicrobial biology.organism_classification chemistry Biofilms lcsh:Q Nuclear chemistry |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Mature biofilms are highly resistant to antimicrobial agents due to the presence of extracellular polymeric substances (EPS), which inhibit the penetration of external molecules. In this study, we developed a coordination compound consisting of zinc chloride and erythritol that exhibits penetrating and bactericidal activity against Streptococcus mutans biofilms. An in vitro biofilm model was established in microplates, and bactericidal activity against biofilms was evaluated using an Alamar blue assay. The cause of the antimicrobial activity of the zinc-erythritol mixture on mature biofilms was demonstrated using fast atom bombardment-mass spectrometry, confocal laser scanning microscopy and atomic force microscopy. We demonstrated that zinc chloride spontaneously formed cationic complexes with erythritol in water. The zinc-erythritol complexes reduced intra- and inter-molecular interactions between bacterial exopolysaccharides, a major component of EPS. This activity was confirmed by measuring the attenuation of the hardness of dried polysaccharides isolated from S. mutans biofilms. The reduction in the interactions between polysaccharides allowed the complexes to penetrate into biofilms and kill the embedded bacteria. While approximately 13% of biofilm-associated microbes were killed by a 10 min treatment with 6.6 mM zinc chloride, 45% were killed when a solution containing 19.8 mM erythritol and 6.6 mM zinc chloride was used. This strategy of leveraging the coordination properties of metal ions with sugar alcohols provides a simple way to effectively remove mature biofilms using only conventional substances without the need for intricate chemical synthesis processes. |
| Databáze: | OpenAIRE |
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