Sophisticated mesh filtration technique of a large-scale isolation of islets and their function
| Autor: | Y. Hara, Akimitsu Tsutou, Shigeaki Baba, Kyoya Narutaki, K. Ishihara, Kazushige Ejiri, Hiroshi Taniguchi |
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| Rok vydání: | 1989 |
| Předmět: |
Male
endocrine system medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment Cell Separation Inferior vena cava Islets of Langerhans Endocrinology Internal medicine medicine.artery Diabetes mellitus Internal Medicine medicine Animals Insulin Incubation geography Aorta geography.geographical_feature_category business.industry Rats Inbred Strains General Medicine Islet medicine.disease Rats Transplantation medicine.vein Collagenase business medicine.drug |
| Zdroj: | Diabetes research and clinical practice. 6(2) |
| ISSN: | 0168-8227 |
| Popis: | A large-scale isolation of islets is required for islet transplantation. We improved our conventional method, and could obtain about three times more islets than by the conventional methods. Pancreata of adult Wistar rats were inflated by injection of buffer with (A) or without 1.3 mg/ml collagenase (B). The rats were bled from the inferior vena cava and the aorta in (A) simultaneously with the inflation. They were further digested with collagenase and filtered through two different meshes (pore size: 1190 and 590 μm) (A1) or three different meshes (pore size: 1190, 590 and 120 μm) (A2) in order. Insulin released from islets isolated in this manner was determined by 1-h incubation with 3.3 and 16.7 mM glucose. Besides, 600 islets each were transplanted into the liver of streptozotocin-induced diabetic Wistar rats and their fasting plasma glucose was measured at weekly intervals. (1) With these methods more numerous islets were harvested by A1 (mean: 554) and A2 (mean: 746) than B (mean: 224). (2) Insulin released at both glucose concentrations was similar among islets obtained by A1, A2 and B. (3) The plasma glucose-lowering effect was similar among the islets obtained by these methods. (4) A more selected range of islet sizes was obtained by A2 than A1. These observations indicate that the present techniques (A1 and A2) are less time-consuming and simpler for a large-scale isolation of islets. |
| Databáze: | OpenAIRE |
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