Involvement of TLR4/ CXCL9/ PREX-2 pathway in the development of hepatocellular carcinoma (HCC) and the promising role of early administration of lactobacillus plantarum in Wistar rats
| Autor: | Enas Samir Nabih, Ghada Galal Hamam, Asmaa M. Elshaer, Abeer A. Abd El Samad, Omnyah A. El-Kharashi, Yosra M. Magdy |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cirrhosis Carcinoma Hepatocellular Pharmacology Thioacetamide Liver Cirrhosis Experimental Chemokine CXCL9 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Rats Wistar medicine.diagnostic_test biology Microbiota Probiotics Liver Neoplasms food and beverages Cell Biology General Medicine biology.organism_classification medicine.disease Proliferating cell nuclear antigen Rats Toll-Like Receptor 4 030104 developmental biology medicine.anatomical_structure chemistry Liver Hepatocyte Hepatocellular carcinoma biology.protein 030211 gastroenterology & hepatology Liver function Liver function tests Lactobacillus plantarum Developmental Biology Signal Transduction |
| Zdroj: | Tissuecell. 60 |
| ISSN: | 1532-3072 |
| Popis: | Abstarct Background and aim Improvement of gut microbiota may help in preventing the progression of cirrhosis. We supposed that Lactobacillus Plantarum (L. Plantarum) protects the cirrhotic liver through suppression of TLR4/ CXCL9/ PREX-2. Methodology Rats were divided into two groups. Group I, lasts for six weeks and Group II lasts for 12 weeks. Each group was subdivided into: naive, Lactobacillus Plantarum (L. Plantarum), thioacetamide (TAA) and TAA + L. Plantarum. Liver function tests, α fetoprotein (AFP) levels, CXCL9, PREX-2 and TLR4 expression were assessed. Histological studies were performed. Results TAA induced significant deterioration in liver functions and increased AFP. There was periportal cirrhosis, vacuolated hepatocytes, decrease hepatocyte parrafin-1 (hep par-1) expression, increase proliferating cell nuclear antigen (PCNA) positive nuclei and cytokeratin AE1/AE3. The PCR results showed significant increase in TLR4, CXCL9 and PREX-2 expression. Early administration of L. Plantarum significantly decreased the expression of TLR4, CXCL9 and PREX-2 together with improvement in liver function and prevented the pathological changes. Conclusions The cirrhotic complications induced by TAA are through activation of TLR4/ CXCL9/ PREX-2 pathway and could be prevented by the early administration of L. Plantarum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect