Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells
| Autor: | Weiwei Liu, Chundi He, Le Qu, Hong Zhu, Xiaoqing Jian, Qing Mao, Zhen-Hai Yang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Skin Neoplasms Cell Down-Regulation Biology 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Neoplasm Invasiveness Serpins Cell Proliferation Oncogene Cell growth Cell Cycle Maspin General Medicine Cell cycle Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Oncology Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer research Carcinoma Squamous Cell A431 cells |
| Zdroj: | Oncology reports. 37(5) |
| ISSN: | 1791-2431 |
| Popis: | Cutaneous squamous cell carcinoma (cSCC) is a common malignant tumor. Mammary serine protease inhibitor (Maspin), a member of serpin family, has been reported as a tumor suppressor in various carcinomas. In this study, we detected the expression level of Maspin in cSCC tissues by real-time PCR and western blotting, and found that Maspin was downregulated in the cSCC tissues compared with the adjacent normal tissues. Moreover, Maspin was stably overexpressed in A431 cells, and CCK-8 assay, colony formation assay, Transwell assay, Hoechst staining and western blotting were carried out to detect the growth, proliferation, invasion, cell cycle and apoptosis of A431 cells. The results revealed that overexpression of Maspin inhibited growth, proliferation, invasion and cell cycle G1/S/G2 transition and enhanced apoptosis of A431 cells. The pro-apoptotic protein cleaved caspase-3, poly(ADP-ribose) polymerase (PARP) and Bax increased, and the anti-apoptotic protein Bcl-2 decreased after Maspin overexpression. Therefore, we demonstrated that Maspin suppressed growth, proliferation and invasion by delaying cell cycle transition and promoting apoptosis in cSCC cells, which may provide new insights for the clinical diagnosis and therapy of cSCC. |
| Databáze: | OpenAIRE |
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