Intracellular [3H]dopamine binding sites in normal and malignant cells: Relationships to cell proliferation
| Autor: | Mary Alejandro, Rebecca R. Gregory, Barry M. Markaverich |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Dopamine
Catechols Biophysics Biology Binding Competitive Biochemistry Receptors Dopamine Mice Cytosol L Cells Phenols Reference Values Cell surface receptor Tumor Cells Cultured medicine Animals Humans Binding site Receptor Molecular Biology Cell Nucleus Dopamine binding Cell growth Eye Neoplasms Uterus Retinoblastoma Cell Biology Rats Kinetics Female Cell Division Intracellular medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 165:391-400 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(89)91083-8 |
| Popis: | Dopamine interaction with target cells undoubtably involves binding to plasma membrane receptors. However, the well documented cell growth inhibitory activity of this catecholamine suggests nuclear regulation. To evaluate this possibility, we determined the intracellular localization and binding of [3H]dopamine in human retinoblastoma (Y-79 cells), normal mouse fibroblasts (LM-cells), and in the rat uterus. Cytosol and purified nuclear preparations devoid of plasma membrane components contained specific, saturable, high affinity (Kd approximately 20 nM) binding sites for [3H]dopamine. The nuclear binding affinity for dopamine, L-dopa, and L-dopa methyl ester correlated with the inhibitory effects of these compounds on cell proliferation, suggesting that intracellular dopamine binding sites may also be involved in cellular response to catecholamines. |
| Databáze: | OpenAIRE |
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