Ultraviolet B radiation modifies circadian time in epidermal skin and in subcutaneous adipose tissue

Autor: Lasse Ylianttila, Erna Snellman, Veera Nikkola, Timo Partonen, Maija E. Miettinen, Mari Grönroos, Piia Karisola, Harri Alenius
Přispěvatelé: Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology, Tampere University, HYKS erva, Päijät-Häme Welfare Consortium, Department of Bacteriology and Immunology, Faculty of Medicine, Medicum, University of Helsinki
Rok vydání: 2018
Předmět:
Male
CRY2
CLOCK GENES
0301 basic medicine
CRY1
Erythema
Circadian clock
0302 clinical medicine
Cryptochrome
MELANOPSIN
Neurologia ja psykiatria - Neurology and psychiatry
Gene expression
Immunology and Allergy
Skin
ERYTHEMA
integumentary system
UV-RADIATION
circadian associated repressor of transcription (CIART)
General Medicine
CLOCK
Female
medicine.symptom
Adult
EXPRESSION
Melanopsin
medicine.medical_specialty
Ultraviolet Rays
Biolääketieteet - Biomedicine
mRNA
Immunology
Subcutaneous Fat
Repressor
Dermatology
Biology
BLUE-LIGHT PHOTORECEPTORS
03 medical and health sciences
Internal medicine
INDUCED DNA-DAMAGE
medicine
Humans
Radiology
Nuclear Medicine and imaging
RNA
Messenger
Circadian rhythm
gene
RHYTHMS
Cryptochromes
030104 developmental biology
Endocrinology
Gene Expression Regulation
CIART
3121 General medicine
internal medicine and other clinical medicine
PIGMENTATION
030217 neurology & neurosurgery
Zdroj: Photodermatology, Photoimmunology & Photomedicine. 35:157-163
ISSN: 1600-0781
0905-4383
DOI: 10.1111/phpp.12440
Popis: Background Recent findings suggest that circadian time regulates cellular functions in the skin and may affect protection against ultraviolet radiation (UVR). It is not known, however, whether UVR through skin directly affects the expression of circadian genes. We investigated the effect of ultraviolet B (UVB) exposure on cryptochrome circadian clock 1 (CRY1), cryptochrome circadian clock 2 (CRY2), and circadian associated repressor of transcription (CIART) genes. Methods Healthy volunteers (n = 12) were exposed to narrow-band UVB radiation of four standard erythemal dose (SED). Epidermal/dermal and subcutaneous adipose tissue samples were obtained by punch biopsies from irradiated and non-irradiated skin 10 cm away from the irradiated site 24 hours after UVB exposure. Gene expression of CRY1, CRY2, and CIART was measured using RT-PCR (TaqMan). Results Ultraviolet B radiation affected mRNA expression in the epidermal/dermal skin and in the subcutaneous adipose tissue. It down-regulated expression of CRY2 gene in the epidermal/dermal skin, whereas it up-regulated expression of CRY1 and CIART genes in the subcutaneous adipose tissue. Conclusion We showed for the first time that UVB radiation affects expression of circadian genes in the subcutaneous adipose tissue. Further studies are warranted to understand the mechanisms in detail.
Databáze: OpenAIRE
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