Entamoeba histolytica: Flavins in axenic organisms
| Autor: | Hsin-sheng Lo, Richard E. Reeves |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Flavin Mononucleotide
Riboflavin Immunology Flavin mononucleotide Flavoprotein Flavin group chemistry.chemical_compound Entamoeba histolytica Animals heterocyclic compounds Lumiflavin Axenic chemistry.chemical_classification biology digestive oral and skin physiology food and beverages General Medicine biology.organism_classification Culture Media Infectious Diseases Enzyme Biochemistry chemistry Flavin-Adenine Dinucleotide biology.protein Parasitology |
| Zdroj: | Experimental Parasitology. 47:180-184 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(79)90071-7 |
| Popis: | The individual flavin species of axenic Entamoeba histolytica were assayed: separated riboflavin was assayed by the lumiflavin method; flavin-adenine dinucleotide (FAD), by an enzymatic method; flavin mononucleotide (FMN) was calculated from the difference, total flavin minus FAD and riboflavin. The amount of flavin in micrograms per grams fresh cells follows: total flavin, 7.6 ± 0.9 calculated as riboflavin; riboflavin, 1.6 ± 0.7; FMN, 6.6 ± 0.5; and FAD, 1.2 ± 0.1. Recalculated to nanomoles per milligrams total amebal protein these values were: total flavin, 0.21; riboflavin, 0.04; FMN, 0.15; and FAD, 0.02. The identity of each flavin was confirmed by a paper chromatographic method. Analyses on Panmede, the main source of flavins in the TP-S-1 medium, indicate that it contains all three forms of flavin. Its contribution to growth medium in micrograms per milliliters: riboflavin, 2.1 ± 0.3; FMN, 0.6 ± 0.1; and FAD, 0.4 ± 0.1. The in vivo biosynthesis of FMN and FAD from riboflavin by E. histolytica is demonstrated. A new and convenient method was found to separate riboflavin from flavin nucleotides in tissue extracts. |
| Databáze: | OpenAIRE |
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