Prevalence of enteric opportunistic pathogens and extended-spectrum cephalosporin- and carbapenem-resistant coliforms and genes in wastewater from municipal wastewater treatment plants in Croatia
| Autor: | Ana Puljko, Milena Milaković, Stela Križanović, Josipa Kosić-Vukšić, Ivana Babić, Ines Petrić, Ana Maravić, Marko Jelić, Nikolina Udiković-Kolić |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Environmental Engineering
Croatia Health Toxicology and Mutagenesis Microbial Sensitivity Tests Wastewater treatment ESBL Carbapenemase Antibiotic resistance genes Enteric opportunistic pathogens Wastewater beta-Lactamases Water Purification 03 medical and health sciences Waste Management Escherichia coli Prevalence Environmental Chemistry Waste Management and Disposal 030304 developmental biology 0303 health sciences 030306 microbiology Molecular Biotechnology Pollution 6. Clean water 3. Good health Anti-Bacterial Agents Cephalosporins Carbapenems Interdisciplinary Technical Sciences Biotechnology |
| Zdroj: | Journal of Hazardous Materials |
| Popis: | Extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are a critical global health problem and wastewater treatment plants (WWTPs) can promote their spread into the environment ; yet their efficacy is not well characterized. Here, we have used conventional culturing to monitor coliform bacteria and quantitative PCR to monitor 2 ESBL and 5 carbapenemase (CP) genes and 4 enteric opportunistic pathogens (EOPs) in the influent and effluent of 7 Croatian WWTPs in two seasons. In general, levels of total, cefotaxime and carbapenem-resistant coliforms were significantly reduced but not eliminated by conventional treatment in most WWTPs. Most WWTPs efficiently removed EOPs such as K. pneumoniae and A. baumannii, while E. coli and Enterococcus spp. were reduced but still present in relatively high concentrations in the effluent. ESBL genes (blaTEM and blaCTX-M-32) were only slightly reduced or enriched after treatment. CP genes, blaKPC-3, blaNDM and blaOXA-48-like, were sporadically detected, while blaIMP and blaVIM were frequently enriched during treatment and correlated with plant size, number or size of hospitals in the catchment area, and COD effluent concentration. Our results suggest that improvements in wastewater treatment technologies are needed to minimize the risk of environmental contamination with top priority EOPs and ARGs and the resulting public health. |
| Databáze: | OpenAIRE |
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