The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration
| Autor: | A. V. Antonov, C. Paicu, Tamas Dalmay, Anna Wilczynska, Jack D. Godfrey, Adam E. Hall, Wei-Ting Lu, Martin Bushell, Simon Moxon, P. A. Muller |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Ankyrins
0301 basic medicine Cancer Research DNA Repair DNA repair DNA damage Blotting Western Immunology Breast Neoplasms Biology Cell Line 03 medical and health sciences Cellular and Molecular Neuroscience Cell Movement ANK1 Humans Protein Isoforms Ankyrin RNA Messenger RNA Small Interfering Cytoskeleton chemistry.chemical_classification Genetics Antibiotics Antineoplastic Manchester Cancer Research Centre ResearchInstitutes_Networks_Beacons/mcrc Signal transducing adaptor protein Cell migration Cell Biology Actin cytoskeleton Up-Regulation Cell biology Actin Cytoskeleton MicroRNAs 030104 developmental biology Microscopy Fluorescence chemistry Doxorubicin Original Article Female RNA Interference Tumor Suppressor Protein p53 DNA Damage |
| Zdroj: | Hall, A E, Lu, W T, Godfrey, J D, Antonov, A V, Paicu, C, Moxon, S, Dalmay, T, Wilczynska, A, Muller, P A J & Bushell, M 2016, ' The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration ', Cell Death and Disease, vol. 7, e2184 . https://doi.org/10.1038/cddis.2016.91 Cell Death & Disease |
| ISSN: | 2041-4889 |
| Popis: | The integrity of the genome is maintained by a host of surveillance and repair mechanisms that are pivotal for cellular function. The tumour suppressor protein p53 is a major component of the DNA damage response pathway and plays a vital role in the maintenance of cell-cycle checkpoints. Here we show that a microRNA, miR-486, and its host gene ankyrin-1 (ANK1) are induced by p53 following DNA damage. Strikingly, the cytoskeleton adaptor protein ankyrin-1 was induced over 80-fold following DNA damage. ANK1 is upregulated in response to a variety of DNA damage agents in a range of cell types. We demonstrate that miR-486-5p is involved in controlling G1/S transition following DNA damage, whereas the induction of the ankyrin-1 protein alters the structure of the actin cytoskeleton and sustains limited cell migration during DNA damage. Importantly, we found that higher ANK1 expression correlates with decreased survival in cancer patients. Thus, these observations highlight ANK1 as an important effector downstream of the p53 pathway. |
| Databáze: | OpenAIRE |
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