Volume holographic optical element for light sheet fluorescence microscopy
| Autor: | Yuan Luo, Sunil Vyas, Jui-Ching Wu, Ting-Yu Hsieh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Holographic grating Optical sectioning business.industry Holographic optical element Holography 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Atomic and Molecular Physics and Optics law.invention 010309 optics Optics law Light sheet fluorescence microscopy 0103 physical sciences 0210 nano-technology business Preclinical imaging Volume (compression) |
| Zdroj: | Optics letters. 45(23) |
| ISSN: | 1539-4794 |
| Popis: | Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky. Here, we propose and demonstrate the application of a volume holographic optical element (photopolymer-based volume holographic grating) for designing a compact LSFM system, called a volume holographic LSFM (VHLSFM). Using the VHLSFM, we performed in vivo imaging of Caenorhabditis elegans (C. elegans) and observed high-contrast optically sectioned fluorescence images of the oocytes and embryonic development in real time for 3D imaging. |
| Databáze: | OpenAIRE |
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