Lidocaine inhibits potassium efflux and hemolysis in erythrocytes during oxidative stress in vitro
| Autor: | Luc Rochette, Marc Freysz, François Volot, J.J. Lahet, Catherine Vergely, François Lenfant |
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| Rok vydání: | 2000 |
| Předmět: |
Antioxidant
Erythrocytes Oxygen radical absorbance capacity Lidocaine Radical Potassium medicine.medical_treatment Amidines chemistry.chemical_element In Vitro Techniques medicine.disease_cause Hemolysis Antioxidants medicine Humans Anesthetics Local Pharmacology Chromatography Electron Spin Resonance Spectroscopy Biological Transport medicine.disease Oxidants Red blood cell Oxidative Stress medicine.anatomical_structure Biochemistry chemistry Oxidative stress medicine.drug |
| Zdroj: | General pharmacology. 34(3) |
| ISSN: | 0306-3623 |
| Popis: | Lidocaine is a widely used local anesthetic agent. The aim of this work was to study the action of lidocaine on human red blood cells exposed to an oxidative stress in vitro. Blood was obtained from healthy volunteers. After separation from plasma, the erythrocytes were suspended in phosphate buffer. Oxidative stress was induced by incubation with a free radical generator, the 2,2' azobis (2-amidinopropane) hydrochloride (AAPH). Erythrocytes were incubated with or without lidocaine at two concentrations (36.93 and 73.85 microM) and with or without AAPH (20 mM). Electron paramagnetic resonance (EPR) spectroscopy was performed to identify the free radical species generated by AAPH using the spin trap 5-5'-dimethyl-L-pyroline-N-oxide (DMPO). Different sets of experiments were run. Potassium efflux was measured by flame photometry in each group at time 0 min and every 30 min of the experiment for 2 h. Hemolysis was studied by the Drabkin method at increasing concentrations of AAPH (20, 50, and 100 mM) and with or without lidocaine (36.93 microM). The oxygen radical absorbance capacity (ORAC) was measured by using allophycocyanin (APC) as a fluorescent indicator protein, and the antioxidant capacity of lidocaine (36.93 microM) was studied by the analysis of fluorescence of the APC. AAPH was shown to produce alkoxyl free radicals. Oxidative stress induced a marked increase in the potassium efflux and the hemolysis that was AAPH dose-dependent. Lidocaine inhibited the potassium efflux and delayed the occurrence of hemolysis. Lidocaine did not show any antioxidant properties for the free radical species generated by AAPH. In this model, lidocaine protects erythrocytes against oxidative stress. This effect is not explained by a free radical scavenging property. The results may be of great interest in clinical practice such as intravenous regional anesthesia or the prevention of ischemia-reperfusion injury. |
| Databáze: | OpenAIRE |
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