Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response
| Autor: | Mark P. Ashe, Leah E. A. Holmes, Salma Ibrahimo |
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| Rok vydání: | 2006 |
| Předmět: |
RNA Caps
Saccharomyces cerevisiae Proteins Molecular Sequence Data Hyphae Bioengineering Biology urologic and male genital diseases Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Eukaryotic translation Pseudohyphal growth Peptide Initiation Factors Gene Expression Regulation Fungal Genetics Protein biosynthesis Initiation factor Amino Acid Sequence RNA Messenger Nuclear Cap-Binding Protein Complex Cell Proliferation Binding Sites EIF4G EIF4E Translation (biology) Yeast Cell biology Eukaryotic Initiation Factor-4E chemistry Protein Biosynthesis hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Yeast. 23:1075-1088 |
| ISSN: | 1097-0061 0749-503X |
| DOI: | 10.1002/yea.1415 |
| Popis: | The eukaryotic translation initiation factor eIF4E is responsible for the recognition of the mRNA cap structure and, as such, plays a key role in the selection of mRNAs for translation. The interaction of eIF4E with the 'multi-adaptor' eIF4G (and thus recruitment of ribosomes to mRNA) can be regulated via competitive binding of 4E-binding proteins (4E-BPs). 4E-BPs have broad functions in cell growth, proliferation and development. We have found that disruption of the genes for either of the yeast 4E-BPs (Eap1p or Caf20p) leads to an inhibition of pseudohyphal growth in the resulting diploid yeast strain following nitrogen limitation. Specific 4E-binding domain mutations destroy the capacity of each 4E-BP gene to complement the non-pseudohyphal phenotype, suggesting that a translational function for the 4E-BPs is important for pseudohyphal growth. In addition, neither of the 4E-BP deletion strains is deficient in global or stress-regulated protein synthesis. However, our evidence reveals that the two 4E-BPs are functionally distinct with regard to pseudohyphal growth. Therefore, this work supports a model where the yeast 4E-BPs are acting on specific mRNAs to facilitate a defined proliferative response to environmental stress in yeast. |
| Databáze: | OpenAIRE |
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