Real-time PCR-based identification of Borrelia burgdorferi sensu lato species in ticks collected from humans in Romania
| Autor: | Fabian Meyer, Ingrid Huber, Christiane Klier, Violeta T. Briciu, Volker Fingerle, Georgiana Coroiu, Andrei Daniel Mihalca, Cecilia Hizo-Teufel, Dumitru Carstina, Doina F. Ţăţulescu, Mihaela Lupse, Daniela Sebah |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Nymph Entomology Ixodes ricinus Tick Real-Time Polymerase Chain Reaction Microbiology Ticks Sensu Borrelia burgdorferi Group Species Specificity Borrelia parasitic diseases Animals Humans Borrelia burgdorferi biology Romania bacterial infections and mycoses biology.organism_classification Haemaphysalis Virology Tick Infestations Infectious Diseases Real-time polymerase chain reaction Tick-Borne Diseases Insect Science Larva Parasitology Female |
| Zdroj: | Ticks and tick-borne diseases. 5(5) |
| ISSN: | 1877-9603 |
| Popis: | The aims of our study were to determine (i) which tick species bite humans in Romania and (ii) the prevalence of Borrelia (B.) burgdorferi genospecies in these ticks. All ticks collected from patients who presented to the Clinic of Infectious Diseases Cluj Napoca in spring/summer 2010 were morphologically identified by an entomologist and tested for B. burgdorferi genospecies prevalence by a real-time PCR assay targeting the hbb gene and melting curve analysis. Out of 532 ticks, 518 were Ixodes ricinus, 10 Dermacentor marginatus, and 3 Haemaphysalis spp. ticks, and one unidentified tick due to destruction. Since evaluation of the hbb PCR revealed that it was not possible to differentiate between B. spielmanii/B. valaisiana and B. garinii/B. bavariensis, sequencing of an 800-bp fragment of the ospA gene was performed in these cases. Out of 389 investigated ticks, 43 were positive by hbb PCR for B. burgdorferi sensu lato. The positive samples were 42 Ixodes ricinus (11.1% B. burgdorferi sensu lato prevalence) and the one unidentified tick. Species identification revealed the presence of mainly B. afzelii, but also of B. garinii, B. burgdorferi sensu stricto, B. valaisiana, and B. lusitaniae. In 4 samples, differentiation between B. spielmanii/B. valaisiana was impossible. Our study shows that the most relevant human pathogenic B. burgdorferi genospecies – predominantly B. afzelii – are present in ticks collected from Romanian patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect