Distinct regions of the human granulocyte-colony-stimulating factor receptor cytoplasmic domain are required for proliferation and gene induction
| Autor: | David P. Gearing, B Mosley, Heinz Baumann, S.F. Ziegler, T A Bird, K. K. Morella |
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| Rok vydání: | 1993 |
| Předmět: |
Transcriptional Activation
Cytoplasm Recombinant Fusion Proteins DNA Mutational Analysis Molecular Sequence Data Biology In Vitro Techniques Transfection Structure-Activity Relationship Liver Neoplasms Experimental Gene expression Tumor Cells Cultured Animals Amino Acid Sequence Receptor Promoter Regions Genetic Peptide sequence Molecular Biology Sequence Deletion Regulation of gene expression chemistry.chemical_classification Genetics Cell Biology Cell biology Amino acid Rats chemistry Gene Expression Regulation Receptors Granulocyte Colony-Stimulating Factor Signal transduction Granulocyte colony-stimulating factor receptor Cell Division Research Article Acute-Phase Proteins Signal Transduction |
| Zdroj: | Molecular and Cellular Biology. 13:2384-2390 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.13.4.2384 |
| Popis: | Using two different cell systems, we show that the cytoplasmic domain of the granulocyte-colony-stimulating factor receptor (G-CSFR) may be composed of at least two functional regions. The first, within the membrane-proximal 57 amino acids, is absolutely required to deliver a proliferative signal. This region contains two sequence motifs conserved between members of the hematopoietin receptor family. The second functional region resides between amino acids 57 and 96. This region is required for the induction of acute-phase plasma protein gene expression when the G-CSFR is transfected into human hepatoma cell lines. The G-CSFR-transfected hepatoma cells respond to G-CSF by increasing the production of the same set of plasma proteins as stimulated by interleukin-6, suggesting that the two cytokines share a common signal transduction pathway. |
| Databáze: | OpenAIRE |
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