Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization
| Autor: | Vesela Lozanova, Florence Debacq-Chainiaux, Catherine Lambert de Rouvroit, Maryse Hermant, Abdallah Mound, Christelle Guéré, Julie Robic, Céline Warnon, Yves Poumay, Donatienne Tyteca, Katell Vié |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Keratinocytes Sphingomyelin Membrane Microdomains/metabolism Biological/metabolism Lipids/physiology Re-Epithelialization/physiology chemistry.chemical_compound Cholesterol/metabolism 0302 clinical medicine Re-Epithelialization Cell Movement Toxins Keratinocyte migration Toxins Biological/metabolism Sphingomyelins/metabolism Lipid bilayer Lipid raft Cells Cultured Cultured Lipids Sphingomyelins Cell biology Membrane Cholesterol lipids (amino acids peptides and proteins) Senescence Cells Biology Membrane Lipids 03 medical and health sciences Membrane Microdomains Keratinocytes/metabolism Journal Article Humans Lipid submicrometric domains Molecular Biology Toxins Biological Cell Membrane/metabolism Cell Membrane Cell Movement/physiology Cell Biology 030104 developmental biology chemistry Membrane Lipids/metabolism mCherry 030217 neurology & neurosurgery |
| Zdroj: | Mound, A, Lozanova, V, Warnon, C, Hermant, M, Robic, J, Guere, C, Vie, K, Lambert de Rouvroit, C, Tyteca, D, Debacq-Chainiaux, F & Poumay, Y 2017, ' Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization ', Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, vol. 1862, no. 9, pp. 958-971 . https://doi.org/10.1016/j.bbalip.2017.06.001 |
| Popis: | Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alters lipid domains and reduce migration ability. |
| Databáze: | OpenAIRE |
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