Ultrasound-Triggered Enzymatic Gelation
| Autor: | Valeria Nele, Carolyn E. Schutt, James P. K. Armstrong, Jonathan P. Wojciechowski, Worrapong Kit-Anan, James Doutch, Molly M. Stevens |
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| Přispěvatelé: | Medical Research Council (MRC), Engineering & Physical Science Research Council (EPSRC), British Heart Foundation, Arthritis Research UK, Medical Research Council |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Technology
CROSS-LINKING Chemistry Multidisciplinary 02 engineering and technology 01 natural sciences 09 Engineering Polyethylene Glycols Calcium Chloride General Materials Science TISSUE TRANSGLUTAMINASE DRUG-DELIVERY Liposome 02 Physical Sciences Microbubbles Chemistry Physical ultrasound Physics Hydrogels 021001 nanoscience & nanotechnology Chemistry Cross-Linking Reagents Physics Condensed Matter Ultrasonic Waves Mechanics of Materials Covalent bond Self-healing hydrogels Drug delivery Physical Sciences Science & Technology - Other Topics 0210 nano-technology 03 Chemical Sciences LIPID VESICLES liposomes Materials science Phosphorylcholine Materials Science enzymes Materials Science Multidisciplinary macromolecular substances 010402 general chemistry complex mixtures Article Catalysis Enzyme catalysis Physics Applied FOCUSED ULTRASOUND Enzyme kinetics Nanoscience & Nanotechnology RELEASE Science & Technology Mechanical Engineering Phosphatidylethanolamines technology industry and agriculture Fibrinogen 0104 chemical sciences Kinetics POLYSACCHARIDE Chemical addition Biophysics |
| Zdroj: | Advanced materials (Deerfield Beach, Fla.) |
| ISSN: | 1521-4095 0935-9648 |
| Popis: | Hydrogels are formed using various triggers, including light irradiation, pH adjustment, heating, cooling or chemical addition. In this report, a new method for forming hydrogels is introduced: ultrasound-triggered enzymatic gelation. Specifically, ultrasound is used as a stimulus to liberate liposomal calcium ions, which then trigger the enzymatic activity of transglutaminase. The activated enzyme catalyzes the formation of fibrinogen hydrogels through covalent intermolecular crosslinking. The catalysis and gelation processes are monitored in real time and both the enzyme kinetics and final hydrogel properties are controlled by varying the initial ultrasound exposure time. This technology is extended to microbubble-liposome conjugates, which exhibit a stronger response to the applied acoustic field and are also used for ultrasound-triggered enzymatic hydrogelation. To the best of our knowledge, these results are the first instance in which ultrasound has been used as a trigger for either enzyme catalysis or enzymatic hydrogelation. This approach is highly versatile and Peer reviewed version of the manuscript published in final form at Advanced Materials (2020) 2 could be readily applied to different ion-dependent enzymes or gelation systems. Moreover, this work paves the way for the use of ultrasound as a remote trigger for in vivo hydrogelation. |
| Databáze: | OpenAIRE |
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