Attachment, Proliferation And Collagen Type I Mrna Expression Of Human Gingival Fibroblasts On Different Biodegradable Membranes
| Autor: | Nuhan Purali, Petek Korkusuz, Mahmut Kus, Sema S. Hakki, Ismet Duran, Buket S. Bozkurt |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Cell Culture Techniques Gingiva Biocompatible Materials macromolecular substances Biochemistry Collagen Type I chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Rheumatology Cell Adhesion medicine Animals Humans Acellular Dermis Gingival Recession Orthopedics and Sports Medicine MTT assay Horses Lactic Acid RNA Messenger Cell adhesion Molecular Biology Gingival recession Cell Proliferation Tissue Engineering Tissue Scaffolds Cell growth Membranes Artificial Cell Biology Anatomy Fibroblasts Molecular biology PLGA Membrane chemistry Cell culture Female medicine.symptom Polyglycolic Acid Type I collagen |
| Popis: | The purpose of this study was to investigate adhesion, proliferation and type I collagen (COL I) mRNA expression of gingival fibroblasts on different membranes used in periodontal applications. Collagen (C), acellular dermal matrix (ADM) and polylactic acid; polyglycolic acid; lactide/glycolide copolymer (PLGA) biodegradable membranes were combined with gingival fibroblasts in culture and incubated for 48 h. Cell adhesion was examined with scanning electron and confocal microscopy. MTT assay was used to measure proliferation. COL I mRNA expression was assessed using quantitative-polymerase chain reaction (QPCR). The PLGA group exhibited the lowest cell survival on day 5 and 10, and lowest cell proliferation on days 5, 10 and 14. While cell proliferation was similar in C and ADM groups, the C membrane showed a slightly greater increase in viable cells to day 10. Confocal and scanning electron microscopy confirmed the results of proliferation and MTT assays. The highest COL I mRNA expression was noted in the PLGA membrane group when compared to the C (p |
| Databáze: | OpenAIRE |
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