Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1 in Escherichia coli recoverd from migrating barnacle geese (Branta Leucopis)
Autor: | Paula Kurittu, Michael S.M. Brouwer, Annamari Heikinheimo, Banafsheh Khakipoor |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
migratory birds 030106 microbiology Virulence extended-spectrum beta-lactamases Antimicrobial resistance medicine.disease_cause 03 medical and health sciences Plasmid Antibiotic resistance multidrug resistance Life Science Medicine One Health Replicon Gene Escherichia coli Host Pathogen Interaction & Diagnostics Genetics Whole genome sequencing whole genome sequencing business.industry Bacteriologie Bacteriology hybrid sequencing Articles Bacteriology Host Pathogen Interaction & Diagnostics biochemical phenomena metabolism and nutrition bacterial infections and mycoses Host Pathogen Interactie & Diagnostiek Multiple drug resistance 030104 developmental biology Bacteriologie Host Pathogen Interactie & Diagnostiek business Research Article |
Zdroj: | Open Research Europe, 1(46) Open Research Europe 1 (2021) 46 Open Research Europe |
ISSN: | 2732-5121 |
Popis: | Background: Increasing antimicrobial resistance (AMR) is a global threat and wild migratory birds may act as mediators of resistant bacteria across country borders. Our objective was to study extended-spectrum beta-lactamase (ESBL) and plasmid-encoded AmpC (pAmpC) producing Escherichia coli in barnacle geese using whole genome sequencing (WGS) and to identify plasmids harboring bla genes. Methods: Barnacle geese feces (n=200) were collected during fall 2017 and spring 2018 from an urban area in Helsinki, Finland. ESBL/AmpC-producing E. coli were recovered from nine samples (4.5%) and isolates were subjected to WGS on both short- and long-read sequencers, enabling hybrid assembly and determination of the genomic location of bla genes. Results: A rare multireplicon IncN+IncR was recovered from one isolate carrying blaCTX-M-1 in addition to aadA2b, lnu(F), and qnrS1. Moreover, rarely detected IncY plasmids in two isolates were found to harbor multiple resistance genes in addition to the human-associated blaCTX-M-15. Poultry-associated blaCMY-2 was identified from the widely distributed IncI1 and IncK plasmids from four different isolates. One isolate harbored an IncI1 plasmid with blaCTX-M-1 and flor. A chromosomal point mutation in the AmpC promoter was identified in one of the isolates. WGS analysis showed isolates carried multiple resistance and virulence genes and harbored multiple different plasmid replicons in addition to bla-carrying plasmids. Conclusions: Our findings suggest that wild migratory birds serve as a limited source of ESBL/AmpC-producing E. coli and may act as disseminators of the epidemic plasmid types IncI1 and IncK but also rarely detected plasmid types carrying multidrug resistance. Human and livestock-associated ESBL enzyme types were recovered from samples, suggesting a potential for interspecies transmission. WGS offers a thorough method for studying AMR from different sources and should be implemented more widely in the future for AMR surveillance and detection. Understanding plasmid epidemiology is vital for efforts to mitigate global AMR spread. |
Databáze: | OpenAIRE |
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