Growth Inhibition of A549 Human Lung Adenocarcinoma Cells by L‐Canavanine Is Associated with p21/WAF1 Induction
| Autor: | Gerald A. Rosenthal, Naoko Ohtani-Fujita, Yi Ding, Yoshizumi Matsukawa, Toshiyuki Sakai, Takaaki Fujii, Daishiro Kato, Yuji Naito, Su Dao, Toshikazu Yoshikawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
p53
Cyclin-Dependent Kinase Inhibitor p21 Cancer Research Lung Neoplasms Tumor suppressor gene Adenocarcinoma Article chemistry.chemical_compound Canavanine Cyclins p21/WAF1 Tumor Cells Cultured Humans Enzyme Inhibitors A549 cell biology Cell growth Kinase L‐Canavanine Cell Cycle Retinoblastoma protein G1 Phase Cell cycle Genes p53 Kinetics Oncology chemistry biology.protein Cancer research cardiovascular system Growth inhibition Tumor Suppressor Protein p53 RB Cell Division |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 1876-4673 0910-5050 |
| Popis: | L-Canavanine (CAV) is a higher plant nonprotein amino acid and a potent L-arginine antimetabolite. CAV can inhibit the proliferation of tumor cells in vitro and in vivo, but little is known regarding the molecular mechanisms mediating these effects. We demonstrated that the treatment of human lung adenocarcinoma A549 cells with CAV caused growth inhibition; G1 phase arrest is accompanied by accumulation of an incompletely phosphorylated form of the retinoblastoma protein, whose phosphorylation is necessary for cell cycle progression from G1 to S phase. In addition, CAV induces the expression of p53 and subsequent expression of a cyclin-dependent kinase inhibitor, p21/WAF1. The p53-dependent induction of p21/WAF1 and the following dephosphorylation of the retinoblastoma protein by CAV could account for the observed CAV-mediated G1 phase arrest. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text