Gas chromatographic method for determining very long chain fatty acids that compose D003 in aqueous suspensions from 20 to 200 mg/ml, used in pharmacological and toxicological studies
| Autor: | Abilio Laguna Granja, Roxana Sierra Pérez, Víctor L González Canavaciolo, Ernesto Méndez Antolín, Yanet Tejeda Díaz, David Marrero Delange |
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| Rok vydání: | 2003 |
| Předmět: |
Chromatography
Gas Aqueous solution Chloroform Chromatography Polymers Fatty Acids Pharmaceutical Science Repeatability Dosage form Saccharum chemistry.chemical_compound Suspensions chemistry Drug Discovery Saturated fatty acid Gas chromatography Plant Structures Derivatization Fatty acid methyl ester |
| Zdroj: | Il Farmaco. 58:521-525 |
| ISSN: | 0014-827X |
| Popis: | D003 is a natural mixture of fatty acids (C(24:0) to C(36:0)), which shows antiplatelet, antithrombotic, and cholesterol-lowering effects in experimental models. A specific gas chromatographic method, using a BPX-5 wide-bore column and 1-nonadecanoic acid as internal standard, was developed and validated to determine the content of D003 in 20-200 mg/ml aqueous suspensions, which are used in pharmacological and toxicological studies. Fatty acids were extracted with chloroform and converted to methyl esters derivatives using 5% aqueous HCl-methanol. The method was linear for suspensions ranging from 10 to 250 mg/ml (correlation coefficient=0.9998) and showed a good accuracy, with average recoveries (98.5-101.28%) no significantly different from 100%, according to the Student t-test (P=0.05). The RSDs were |
| Databáze: | OpenAIRE |
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