The Function of the Broad-Complex During Drosophila melanogaster Oogenesis
| Autor: | George Tzolovsky, Wu-Min Deng, Mary Bownes, Thomas Schlitt |
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| Rok vydání: | 1999 |
| Předmět: |
endocrine system
Heterozygote Biology Oogenesis Gene duplication Gene expression Genetics Animals Drosophila Proteins Protein Isoforms Endoreduplication Gene Alleles Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Homozygote Ovary Gene Expression Regulation Developmental Zinc Fingers biology.organism_classification Molecular biology Drosophila melanogaster embryonic structures Female Ectopic expression Transcription Factors Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/153.3.1371 |
| Popis: | The Broad-Complex (BR-C) is an early ecdysone response gene that functions during metamorphosis and encodes a family of zinc-finger transcription factors. It is expressed in a dynamic pattern during oogenesis. Its late expression in the lateral-dorsal-anterior follicle cells is related to the morphogenesis of the chorionic appendages. All four zinc-finger isoforms are expressed in oogenesis, which is consistent with the abnormal appendage phenotypes resulting from their ectopic expression. We investigated the mechanism by which the BR-C affects chorion deposition by using BrdU to follow the effects of BR-C misexpression on DNA replication and in situ hybridization to ovarian mRNA to evaluate chorion gene expression. Ectopic BR-C expression leads to prolonged endoreplication and to additional amplification of genes, besides the chorion genes, at other sites in the genome. The pattern of chorion gene expression is not affected along the anterior-posterior axis, but the follicle cells at the anterior of the oocyte fail to migrate correctly in an anterior direction when BR-C is misexpressed. We conclude that the target genes of the BR-C in oogenesis include a protein essential for endoreplication and chorion gene amplification. This may provide a link between steroid hormones and the control of DNA replication during oogenesis. |
| Databáze: | OpenAIRE |
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