Synergistic improvements in cell and axonal migration across sciatic nerve lesion gaps using bioresorbable filaments and heregulin-beta1
| Autor: | Robert C. Eberhart, Jie Cai, George M. Smith, Kevin D. Nelson, Xue-Jun Peng |
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| Rok vydání: | 2004 |
| Předmět: |
Silicon
Materials science Polymers Neuregulin-1 Polyesters Biomedical Engineering Schwann cell Biocompatible Materials Microfilament Schwann cell proliferation Biomaterials Cell Movement medicine Animals Lactic Acid Axon Matrigel Schwann cell migration Anatomy Prostheses and Implants Sciatic Nerve Axons Cell biology Nerve Regeneration Rats Drug Combinations medicine.anatomical_structure nervous system Neuregulin Female Proteoglycans Sciatic nerve Collagen Laminin |
| Zdroj: | Journal of biomedical materials research. Part A. 69(2) |
| ISSN: | 1549-3296 |
| Popis: | The success of entubulation for peripheral nerve regeneration is still limited, especially with long lesion gaps. In this study, we examined if regeneration could be en- hanced by constructing implants to both align axonal growth and promote Schwann cell proliferation and migra- tion. Silicone implants were used to bridge a 1.4-cm gap in the rat sciatic nerve. Adult female Sprague-Dawley rats were divided into four groups of tubes containing either 1) Matrigel; 2) Matrigel and heregulin; 3) Matrigel and poly(l- lactic acid) (PLLA) microfilaments; or 4) Matrigel, PLLA microfilaments, and heregulin. Ten weeks postimplantation, the number of axons and Schwann cells were measured at the distal end of implants. Implants with microfilaments displayed better tissue cable formation, increased Schwann cell migration, and regeneration of anti-calcitonin gene-re- lated peptide-positive axons, but not RMDO95-positive ax- ons compared with nonfilament-containing groups. Heregu- lin treatment caused an increase in Schwann cell number, but it demonstrated no significant improvement in either tissue cable formation or axon number. Extensive regenera- tion was observed through implants containing Matrigel, microfilaments, and heregulin, which induced significant improvements in the number and longitudinal organization of both Schwann cells and axons. These results indicate that physical guidance of microfilaments and the Schwann cell growth factor, heregulin, act synergistically to improve nerve regeneration across long lesion gaps. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 247-258, 2004 |
| Databáze: | OpenAIRE |
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