Raman characterization of human skin aging

Autor: Gwendal Josse, Anne-Marie Schmitt, Eléonore Gravier, Emmanuel Questel, Aurélie Villaret, Tuvana Satar, Célia Ipinazar, Valérie Samouillan, Florence Nadal, Céline Mias
Přispěvatelé: Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - INPT (FRANCE), Laboratoires Pierre Fabre (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France), Centre de Recherche sur la Peau [Toulouse], Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Skin Research and Technology
Skin Research and Technology, Wiley, 2018, pp.270-276. ⟨10.1111/srt.12643⟩
ISSN: 0909-752X
1600-0846
DOI: 10.1111/srt.12643⟩
Popis: International audience; Skin aging is a complex biological process mixing intrinsic and extrinsic factors, such as sun exposure. At the molecular level, skin aging affects in particular the extracellular matrix proteins. Materials and Methods: Using Raman imaging, which is a nondestructive approach appropriate for studying biological samples, we analyzed how aging modifies the matrix proteins of the papillary and reticular dermis. Biopsies from the buttock and dorsal forearm of volunteers younger than 30 and older than 60 were analyzed in order to identify chronological and photoaging processes. Analyses were performed on skin section, and Raman spectra were acquired separately on the different dermal layers. Results: We observed differences in dermal matrix structure and hydration state with skin aging. Chronological aging alters in particular the collagen of the papillary dermis, while photoaging causes a decrease in collagen stability by altering proline and hydroxyproline residues in the reticular dermis. Moreover, chronological aging alters glycosaminoglycan content in both dermal compartments. Conclusion: Alterations of the papillary and reticular dermal matrix structures during photo-and chronological aging were clearly depicted by Raman spectroscopy.
Databáze: OpenAIRE