DNA adenine methylation modulates pathogenicity of Klebsiella pneumoniae genotype K1
| Autor: | Shih-Feng Tsai, Chi-Tai Fang, Wen-Ching Yi, Chia-Tung Shun |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Microbiology (medical) Blood Bactericidal Activity Site-Specific DNA-Methyltransferase (Adenine-Specific) bacterial pathogenesis Genotype Klebsiella pneumoniae 030106 microbiology Mutant lcsh:QR1-502 Virulence DNA adenine methylation Biology lcsh:Microbiology Microbiology Epigenesis Genetic Lethal Dose 50 03 medical and health sciences chemistry.chemical_compound Gene Knockout Techniques Immunology and Microbiology(all) Animals Immunology and Allergy Gene Mice Inbred BALB C General Immunology and Microbiology Adenine Wild type genotype K1 General Medicine Gene Expression Regulation Bacterial DNA Methylation biology.organism_classification Klebsiella Infections Disease Models Animal 030104 developmental biology Infectious Diseases chemistry DNA methylation virulence regulation DNA |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 50, Iss 4, Pp 471-477 (2017) |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2015.08.022 |
| Popis: | Background/Purpose: Klebsiella pneumoniae genotype K1 is a highly virulent pathogen that causes liver abscess and metastatic endophthalmitis/meningitis. Whether its pathogenicity is controlled by DNA adenine methylase (Dam), an epigenetic regulator of bacterial virulence gene expression, is yet unknown. We aimed to study the role of DNA adenine methylation in the pathogenicity of K. pneumoniae genotype K1. Methods: We identified the dam gene in the prototype tissue-invasive strain (NTUH-K2044) of K. pneumoniae genotype K1, using the strain's complete genome sequence in GenBank. We constructed a dam− mutant and compared it with the wild type, in terms of in vitro serum resistance and in vivo BALB/cByl mice inoculation. Results: Loss of Dam activity in the mutant was verified by MboI restriction digestion of the genomic DNA and a 1000-fold increase in spontaneous mutation rate. The dam mutant lost at least 68% of serum resistance when compared with the wild type (survival ratio at 1 hour: 2.6 ± 0.4 vs. 8.2 ± 1.9; at 2 hours: 3.9 ± 1.6 vs. 17.4 ± 3.6; p values |
| Databáze: | OpenAIRE |
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