Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication
| Autor: | Sarah J. Fletcher, Ok Sarah Shin, Mukesh Kumar, Seong Wook Seo, Rak Kyun Seong, Neil V. Morgan, Young Ki Choi, Ji Ae Kim |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Herpesvirus 3 Human Receptors Retinoic Acid viruses Immunology Biology Virus Replication medicine.disease_cause Article Virus Mice 03 medical and health sciences Immune system Orthomyxoviridae Infections Viral entry Interferon Endoribonucleases Influenza Human medicine Animals Humans Immunology and Allergy RNA Small Interfering Viral shedding Infection Control Gene knockdown Macrophages Immunity Varicella zoster virus virus diseases Epithelial Cells Hematology Virology RAW 264.7 Cells 030104 developmental biology Gene Expression Regulation Viral replication A549 Cells Influenza A virus Varicella Zoster Virus Infection RNA Helicases Signal Transduction medicine.drug |
| Zdroj: | Immunobiology. 222:979-988 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/j.imbio.2017.07.002 |
| Popis: | Schlafen (SLFN) proteins have been suggested to play important functions in cell proliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhanced viral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-β signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses. |
| Databáze: | OpenAIRE |
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