Proteomic Analysis of the Human Tankyrase Protein Interaction Network Reveals Its Role in Pexophagy
| Autor: | Han Han, Wenqi Wang, Nan Li, Albert Paul Ta, Junjie Chen, Mao Tian Zhou, Xu Li, Bing Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nicotinamide adenine dinucleotide Biology Tankyrase-1 Proteomics tankyrase General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound proteomics Interaction network Peroxisomes Humans peroxisome lcsh:QH301-705.5 Polymerase chemistry.chemical_classification Tankyrases Peroxisome pexophagy Cell biology 030104 developmental biology Enzyme HEK293 Cells chemistry lcsh:Biology (General) biology.protein Function (biology) |
| Zdroj: | Cell Reports, Vol 20, Iss 3, Pp 737-749 (2017) Li, X; Han, H; Zhou, M-T; Yang, B; Ta, AP; Li, N; et al.(2017). Proteomic Analysis of the Human Tankyrase Protein Interaction Network Reveals Its Role in Pexophagy. CELL REPORTS, 20(3), 737-749. doi: 10.1016/j.celrep.2017.06.077. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2r75z5rm |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2017.06.077. |
| Popis: | Tankyrase 1 (TNKS) and tankyrase 2 (TNKS2) belong to the poly(ADP-ribose) polymerase family of proteins, which use nicotinamide adenine dinucleotide to modify substrate proteins with ADP-ribose modifications. Emerging evidence has revealed the pathological relevance of TNKS and TNKS2, and identified these two enzymes as potential drug targets. However, the cellular functions and regulatory mechanisms of TNKS/2 are still largely unknown. Through a proteomic analysis, we defined the protein-protein interaction network for human TNKS/2 and revealed more than 100 high-confidence interacting proteins with numerous biological functions in this network. Finally, through functional validation, we uncovered a role for TNKS/2 in peroxisome homeostasis and determined that this function is independent of TNKS enzyme activities. Our proteomic study of the TNKS/2 protein interaction network provides a rich resource for further exploration of tankyrase functions in numerous cellular processes. |
| Databáze: | OpenAIRE |
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