Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio)
| Autor: | Andrew R. Cossins, Luciane V. Mello, M. Peters, Philip Brownridge, Phillip D. Whitfield, Iain S. Young, Amy J. Claydon, Lynn McLean |
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| Rok vydání: | 2008 |
| Předmět: |
Gene isoform
medicine.medical_specialty Carps Physiology chemistry.chemical_element Aquatic Science Calcium Biology Calcium in biology Common carp Calcium-binding protein Internal medicine medicine Animals Protein Isoforms Carp Molecular Biology Ecology Evolution Behavior and Systematics Gene Expression Profiling Skeletal muscle biology.organism_classification Cell biology Biomechanical Phenomena Endocrinology medicine.anatomical_structure Parvalbumins chemistry Insect Science Muscle Fibers Fast-Twitch biology.protein Animal Science and Zoology sense organs Parvalbumin |
| Zdroj: | The Journal of experimental biology. 212(Pt 2) |
| ISSN: | 0022-0949 |
| Popis: | SUMMARYThe mechanical properties of the axial muscles vary along the length of a fish's body. This variation in performance correlates with the expression of certain muscle proteins. Parvalbumin (PARV) is an important calcium binding protein that helps modulate intracellular calcium levels which set the size and shape of the muscle calcium transient. It therefore has a central role in determining the functional properties of the muscle. Transcript data revealed eight specific isoforms of PARV in common carp (Cyprinus carpio)skeletal muscle which we classified as α1 and β1–7. This study is the first to show expression of all eight skeletal muscle PARV isoforms in carp at the protein level and relate regional differences in expression to performance. All of the PARV isoforms were characterised at the protein level using 2D-PAGE and tandem mass spectrometry. Comparison of carp muscle from different regions of the fish revealed a higher level of expression of PARV isoforms β4 and β5 in the anterior region, which was accompanied by an increase in the rate of relaxation. We postulate that changes in specific PARV isoform expression are an important part of the adaptive change in muscle mechanical properties in response to varying functional demands and environmental change. |
| Databáze: | OpenAIRE |
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