Expression of adipocytokines in heart fat depots depending on the degree of coronary artery atherosclerosis in patients with coronary artery disease

Autor: Anastasia A. Kuzmina, Danil Yu Naumov, Maxim Yu. Sinitsky, Yulia Dyleva, E I Palicheva, E. E. Bychkova, E V Belik, Viktoriya N Karetnikova, Olga Gruzdeva, Olga Barbarash, E. V. Fanaskova, D A Borodkina
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Leptin
Physiology
Biopsy
Peptide Hormones
Adipose tissue
Gene Expression
Coronary Artery Disease
030204 cardiovascular system & hematology
Cardiovascular Medicine
Coronary Angiography
Severity of Illness Index
Biochemistry
Vascular Medicine
Coronary artery disease
chemistry.chemical_compound
0302 clinical medicine
Medical Conditions
Animal Cells
Adipocyte
Immune Physiology
Gene expression
Adipocytes
Medicine and Health Sciences
Coronary Heart Disease
Connective Tissue Cells
Innate Immune System
Multidisciplinary
General Medicine
Middle Aged
Coronary Vessels
medicine.anatomical_structure
Adipose Tissue
Connective Tissue
Cardiovascular Diseases
Cardiology
Cytokines
Medicine
Female
Adiponectin
medicine.symptom
Anatomy
Cellular Types
Pericardium
Artery
Research Article
medicine.medical_specialty
Science
Immunology
Adipokine
Lesion
03 medical and health sciences
Signs and Symptoms
Adipokines
Internal medicine
medicine
Genetics
Humans
Aged
Retrospective Studies
business.industry
Biology and Life Sciences
Cell Biology
Molecular Development
medicine.disease
Atherosclerosis
Hormones
030104 developmental biology
Biological Tissue
chemistry
Immune System
Lesions
Clinical Medicine
business
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 3, p e0248716 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Background. In coronary artery disease, a change in the adipocytokine content of local fat depots of the heart is observed. However, it has not yet been established whether the expression levels of the studied parameters are really related to the degree of atherosclerotic lesion of the spacecraft. Aims to identify the features of the expression of adiponectin, leptin and IL-6 by adipocytes of epicardial, perivascular and subcutaneous adipose tissue depending on the degree of atherosclerotic lesion of the coronary channel in coronary heart disease. Materials and methods. The study conducted at the Research Institute for Complex Issues of Cardiovascular Disease in 20172020, included 84 patients with coronary heart disease (CAD), of which 39 with a moderate degree of atherosclerotic lesion of the coronary artery (CA) ( 22 points on the SYNTAX Score scale), 20 with severe (2331 points) and 25 with extremely severe ( 32 points). Biopsies of subcutaneous (SAT), epicardial (EAT) and perivascular adipose tissue (PVAT) were obtained during elective coronary artery bypass grafting (CABG). The expression of adipocytokine genes was determined using polymerase chain reaction (real-time PCR) using TaqMan probes and the concentration of the studied adipocytokines in adipocyte culture medium by enzyme immunoassay. Statistical analysis was performed using Statistica 9.0, a one-dimensional and multi-dimensional logistic regression analysis. Results. In CAD in adipocytes of the cardiac fat depot, a shift in the balance of adipocytokines is observed towards increased expression and secretion of leptin, IL-6 and a decrease in adiponectin with maximum manifestation in severe and extremely severe coronary lesions. Adipocytes of EAT were characterized by the minimal expression of the adiponectin gene against the background of the maximum leptin and IL-6 in comparison with adipocytes SAT and PVAT. Conclusions. Low expression of the adiponectin in EAT and PVAT against the background of increased expression of leptin and IL-6 is associated with an increase in the degree of atherosclerotic lesion of the coronary channel.
Databáze: OpenAIRE
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