Expression of adipocytokines in heart fat depots depending on the degree of coronary artery atherosclerosis in patients with coronary artery disease
Autor: | Anastasia A. Kuzmina, Danil Yu Naumov, Maxim Yu. Sinitsky, Yulia Dyleva, E I Palicheva, E. E. Bychkova, E V Belik, Viktoriya N Karetnikova, Olga Gruzdeva, Olga Barbarash, E. V. Fanaskova, D A Borodkina |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Male Leptin Physiology Biopsy Peptide Hormones Adipose tissue Gene Expression Coronary Artery Disease 030204 cardiovascular system & hematology Cardiovascular Medicine Coronary Angiography Severity of Illness Index Biochemistry Vascular Medicine Coronary artery disease chemistry.chemical_compound 0302 clinical medicine Medical Conditions Animal Cells Adipocyte Immune Physiology Gene expression Adipocytes Medicine and Health Sciences Coronary Heart Disease Connective Tissue Cells Innate Immune System Multidisciplinary General Medicine Middle Aged Coronary Vessels medicine.anatomical_structure Adipose Tissue Connective Tissue Cardiovascular Diseases Cardiology Cytokines Medicine Female Adiponectin medicine.symptom Anatomy Cellular Types Pericardium Artery Research Article medicine.medical_specialty Science Immunology Adipokine Lesion 03 medical and health sciences Signs and Symptoms Adipokines Internal medicine medicine Genetics Humans Aged Retrospective Studies business.industry Biology and Life Sciences Cell Biology Molecular Development medicine.disease Atherosclerosis Hormones 030104 developmental biology Biological Tissue chemistry Immune System Lesions Clinical Medicine business 030217 neurology & neurosurgery Developmental Biology |
Zdroj: | PLoS ONE, Vol 16, Iss 3, p e0248716 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Background. In coronary artery disease, a change in the adipocytokine content of local fat depots of the heart is observed. However, it has not yet been established whether the expression levels of the studied parameters are really related to the degree of atherosclerotic lesion of the spacecraft. Aims to identify the features of the expression of adiponectin, leptin and IL-6 by adipocytes of epicardial, perivascular and subcutaneous adipose tissue depending on the degree of atherosclerotic lesion of the coronary channel in coronary heart disease. Materials and methods. The study conducted at the Research Institute for Complex Issues of Cardiovascular Disease in 20172020, included 84 patients with coronary heart disease (CAD), of which 39 with a moderate degree of atherosclerotic lesion of the coronary artery (CA) ( 22 points on the SYNTAX Score scale), 20 with severe (2331 points) and 25 with extremely severe ( 32 points). Biopsies of subcutaneous (SAT), epicardial (EAT) and perivascular adipose tissue (PVAT) were obtained during elective coronary artery bypass grafting (CABG). The expression of adipocytokine genes was determined using polymerase chain reaction (real-time PCR) using TaqMan probes and the concentration of the studied adipocytokines in adipocyte culture medium by enzyme immunoassay. Statistical analysis was performed using Statistica 9.0, a one-dimensional and multi-dimensional logistic regression analysis. Results. In CAD in adipocytes of the cardiac fat depot, a shift in the balance of adipocytokines is observed towards increased expression and secretion of leptin, IL-6 and a decrease in adiponectin with maximum manifestation in severe and extremely severe coronary lesions. Adipocytes of EAT were characterized by the minimal expression of the adiponectin gene against the background of the maximum leptin and IL-6 in comparison with adipocytes SAT and PVAT. Conclusions. Low expression of the adiponectin in EAT and PVAT against the background of increased expression of leptin and IL-6 is associated with an increase in the degree of atherosclerotic lesion of the coronary channel. |
Databáze: | OpenAIRE |
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