Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values
Autor: | Yinglong Cao, Wenqiong Wang, Shi Zhong, Yu Tian, Lijiang Xuan, Xin Du, Jianxun Zhu, Yougui Li, Jine Chen, Jinxi Huo, Yuqing Sun, Chongming Wu |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Candidate gene Phellinus Genome sequencing Genome DNA sequencing Transcriptome 03 medical and health sciences 0302 clinical medicine Phenylpropanoids biosynthesis lcsh:Science (General) Anti-carcinogenesis activity Gene Genetics Whole genome sequencing lcsh:R5-920 Inoscavin A Multidisciplinary biology food and beverages Phellinus gilvus biology.organism_classification HR-ESI-MS 030104 developmental biology 030220 oncology & carcinogenesis lcsh:Medicine (General) lcsh:Q1-390 |
Zdroj: | Journal of Advanced Research, Vol 24, Iss, Pp 325-335 (2020) |
ISSN: | 2090-1232 |
Popis: | Phellinus gilvus (Schwein.) Pat, a species of ‘Sanghuang’, has been well-documented for various medicinal uses, but the genome information and active constituents are largely unknown. Here, we sequenced the whole-genome of P. gilvus, identified phenylpropanoids as its key anti-cancer components, and deduced their biosynthesis pathways. A 41.11-Mb genome sequence was assembled and the heatmap created with high-throughput chromosome conformation capture techniques data suggested all bins could be clearly divided into 11 pseudochromosomes. Cellular experiments showed that P. gilvus fruiting body was more effective to inhibit hepatocellular carcinoma cells than mycelia. High resolution electrospray ionization mass spectroscopy (HR-ESI-MS) analysis revealed P. gilvus fruiting body was rich in phenylpropanoids, and several unique phenylpropanoids in Phellinus spp. exhibited potent anti-carcinogenesis activity. Based on genomic, HR-ESI-MS information and differentially expressed genes in transcriptome analysis, we deduced the biosynthesis pathway of four major phenylpropanoids in P. gilvus. Transcriptome analysis revealed the deduced genes expressions were synergistically changed with the production of phenylpropanoids. The optimal candidate genes of phenylpropanoids’ synthesis pathway were screened by molecular docking analysis. Overall, our results provided a high-quality genomic data of P. gilvus and inferred biosynthesis pathways of four phenylpropanoids with potent anti-carcinogenesis activities. These will be a valuable resource for further genetic improvement and effective use of the P. gilvus. |
Databáze: | OpenAIRE |
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