Supplemental Figure 1 from Mitochondrial Genomic Backgrounds Affect Nuclear DNA Methylation and Gene Expression

Autor: Danny R. Welch, Bodour Salhia, Gerald C. Gooden, Christophe Legendre, Devin C. Koestler, Stefan Graw, Amanda E. Brinker, Carolyn J. Vivian
Rok vydání: 2023
Popis: Supplemental Figure 1. Validation of Methyl-Seq and RNA-Seq. Several hypo-/hyper-methylated and down-/up-regulated genes were selected for validation using methylation specific primers (64) or quantitative PCR, respectively. Graphs depict fold-change between MNX strains and corresponding wild-type mice are shown to the left. All gene expression data were normalized to beta actin. Each bar combines pools A and B and the experiments were performed in triplicate. Error bars represent standard error. Representative gels showing unmethylated (U) and methylated (M) DNA are shown. The table summarizes specific genes tested and whether the RNA-or Methyl-Seq data were and were not validated.
Databáze: OpenAIRE