(E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol inhibits growth of colon tumors in mice
| Autor: | Jeong Soon Choi, Mi Hee Park, Benjamin Gann, Hae Deun Kim, Dong Ju Son, Kevin X. Rodriguez, Sang-Bae Han, Jin Tae Hong, Jie Zheng, Young Wan Ham, Min Gi Choi, Kyung Tak Nam |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
STAT3 Transcription Factor Small interfering RNA Time Factors Allyl compound Mice Nude Antineoplastic Agents Apoptosis Transfection NF-κB Arsenicals STAT3 chemistry.chemical_compound Phenols In vivo Antineoplastic Combined Chemotherapy Protocols Animals Humans Phenylarsine oxide fas Receptor Receptor Receptors Tumor Necrosis Factor Member 25 Cell Proliferation Mice Inbred BALB C biology Dose-Response Relationship Drug Cell growth NF-kappa B p50 Subunit HCT116 Cells Molecular biology Xenograft Model Antitumor Assays Cyclic S-Oxides Tumor Burden Allyl Compounds Molecular Docking Simulation Oncology chemistry colon cancer Colonic Neoplasms biology.protein death receptor RNA Interference Research Paper Protein Binding Signal Transduction |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Jie Zheng 1,* , Mi Hee Park 1,* , Dong Ju Son 1 , Min Gi Choi 1 , Jeong Soon Choi 1 , Kyung Tak Nam 1 , Hae Deun Kim 1 , Kevin Rodriguez 2 , Benjamin Gann 2 , Young Wan Ham 2 , Sang Bae Han 1 and Jin Tae Hong 1 1 College of Pharmacy and Medical Research Center, Chungbuk National University, Heungduk-gu, Cheongju, Chungbuk, Republic of Korea 2 Department of Chemistry, Utah Valley University, Orem, UT, USA * These authors have contributed equally to this work Correspondence to: Jin Tae Hong, email: // Sang Bae Han, email: // Keywords : colon cancer, apoptosis, STAT3, NF-κB, death receptor Received : June 08, 2015 Accepted : August 31, 2015 Published : October 14, 2015 Abstract In our previous study, we found that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal showed anti-cancer effect, but it showed lack of stability and drug likeness. We have prepared several (E)-2,4-bis(p-hydroxyphenyl)-2-butenal analogues by Heck reaction. We selected two compounds which showed significant inhibitory effect of colon cancer cell growth. Thus, we evaluated the anti-cancer effects and possible mechanisms of one compound (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol in vitro and in vivo . In this study, we found that (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol induced apoptotic cell death in a dose dependent manner (0-15 μg/ml) through activation of Fas and death receptor (DR) 3 in HCT116 and SW480 colon cancer cell lines. Moreover, the combination treatment with (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol and nuclear factor κB (NF-κB) inhibitor, phenylarsine oxide (0.1 μM) or signal transducer and activator of transcription 3 (STAT3) inhibitor, Stattic (50 μM) increased the expression of Fas and DR3 more significantly. In addition, (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol suppressed the DNA binding activity of both STAT3 and NF-κB. Knock down of STAT3 or NF-κB p50 subunit by STAT3 small interfering RNA (siRNA) or p50 siRNA magnified (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol-induced inhibitory effect on colon cancer cell growth. Besides, the expression of Fas and DR3 was increased in STAT3 siRNA or p50 siRNA transfected cells. Moreover, docking model and pull-down assay showed that (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol directly bound to STAT3 and NF-κB p50 subunit. Furthermore, (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol inhibited colon tumor growth in a dose dependent manner (2.5 mg/kg-5 mg/kg) in mice. Therefore, these findings indicated that (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol may be a promising anti-cancer agent for colon cancer with more advanced research. |
| Databáze: | OpenAIRE |
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