Colorimetric determination of hyaluronate degraded byStreptomyces hyaluronidase
| Autor: | Akira Makita, Yoshio Hatae |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Time Factors
Biophysics Hyaluronoglucosaminidase Biochemistry Streptomyces Glycosaminoglycan Hyaluronidase Synovial Fluid medicine Humans Synovial fluid Hyaluronic Acid Colorimetry Molecular Biology Glycosaminoglycans chemistry.chemical_classification Chromatography biology Chemistry Hexosamines Cell Biology Mucopolysaccharidoses biology.organism_classification Enzyme Synovial fluid analysis Protein Binding medicine.drug |
| Zdroj: | Analytical Biochemistry. 64:30-36 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(75)90400-5 |
| Popis: | A novel method for determination of hyaluronate usingStreptomyces hyaluronidase is described. Optimum conditions for the enzymatic reaction were determined. It was not necessary to separate hyaluronate from other glycosaminoglycans. The method has been successfully applied to the determination of hyaluronate in urine and synovial fluid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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