The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm
| Autor: | Agnieszka Pietrosiuk, Helmut Wiedenfeld, E. Jaroszyk, Katarzyna Sykłowska-Baranek, Mirosława Furmanowa, R. Wolinowska |
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| Rok vydání: | 2004 |
| Předmět: |
Rhizobiaceae
Isobutyrylshikonin biology Agrobacterium Alkaloid Lithospermum Plant Science General Medicine Boraginaceae biology.organism_classification Plant Roots Lithospermum canescens Gibberellins chemistry.chemical_compound chemistry Botany Plant biochemistry Pyrrolizidine Spectrophotometry Ultraviolet Biomass Agronomy and Crop Science Chromatography High Pressure Liquid Pyrrolizidine Alkaloids Naphthoquinones |
| Zdroj: | Plant cell reports. 25(10) |
| ISSN: | 0721-7714 |
| Popis: | Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g(-1) DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g(-1) DW) and IBS (0.307 mg g(-1) DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots. |
| Databáze: | OpenAIRE |
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