Analysis of T-cell receptor Vβ gene from infiltrating T cells in insulitis and myocarditis in encephalomyocarditis virus-infected BALB/C mice
| Autor: | Aki Kawagishi, Toshiharu Hayashi, Akikazu Sakudo, Yoshitsugu Matsumoto, Atsutaka Kubosaki, Keiichi Saeki, Natsumi Takeyama, Takashi Onodera |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Encephalomyelitis Autoimmune Experimental Receptors Antigen T-Cell alpha-beta T-Lymphocytes T cell Encephalomyelitis Biophysics Autoimmunity chemical and pharmacologic phenomena medicine.disease_cause Autoantigens Polymerase Chain Reaction Biochemistry Virus BALB/c Mice Antigen Mice Inbred NOD medicine Animals Cloning Molecular Encephalomyocarditis virus Pancreas Molecular Biology Mice Inbred BALB C Models Genetic biology Myocardium T-cell receptor hemic and immune systems Sequence Analysis DNA Cell Biology Glucose Tolerance Test medicine.disease biology.organism_classification Virology Genes T-Cell Receptor Myocarditis medicine.anatomical_structure RNA Insulitis |
| Zdroj: | Biochemical and Biophysical Research Communications. 310:791-795 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2003.09.078 |
| Popis: | Encephalomyocarditis (EMC) virus induces insulin-dependent diabetes and myocarditis in several strains of mice. The T-cell receptor (TCR) Vbeta genes of infiltrating T cells in the pancreas and myocardium of BALB/C mice infected with EMC virus D-variant (EMC-D virus) were analyzed. Using a nested two-step polymerase chain reaction (PCR), TCR Vbeta cDNAs were cloned and sequenced. Two and four kinds of TCR Vbeta clones were obtained from T cells infiltrating into the pancreas and myocardium of BALB/C mice infected with EMC-D virus, respectively. The infiltrating lymphocytes in the diabetic mice expressed Vbeta 8.1, 8.2, and 8.3 genes predominantly. Previously, the use of Vbeta 8.2 has been reported in autoimmune diseases such as murine experimental allergic encephalomyelitis (EAE) and non-obese diabetic (NOD) mouse. This study suggests that mice infected with EMC virus are a useful animal model for autoimmune diseases such as insulin-dependent diabetes. |
| Databáze: | OpenAIRE |
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