Patterns of intracellular cytokines in CD4 and CD8 T cells from patients with mycobacterial infections
| Autor: | K.C. Pereira, Jorgenilce S. Sales, K. S. Cunha, Euzenir Nunes Sarno, Eliane Barbosa de Oliveira, Paulo R. Z. Antas, Elizabeth P. Sampaio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytoplasm Physiology medicine.medical_treatment T cell Immunology Biophysics Biology CD8-Positive T-Lymphocytes Tuberculin Biochemistry IFN-gamma Interferon-gamma Immune system Leprosy medicine Cytotoxic T cell Humans Tuberculosis Interferon gamma General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Tuberculosis Pulmonary Interleukin 4 lcsh:R5-920 Tumor Necrosis Factor-alpha Anti-hCD28 mAb General Neuroscience Cell Biology General Medicine Flow Cytometry Interleukin-10 Mycobacterium leprae Interleukin 10 Cytokine medicine.anatomical_structure lcsh:Biology (General) Cytokines Interleukin-4 lcsh:Medicine (General) CD8 medicine.drug TNF-alpha |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 37, Issue: 8, Pages: 1119-1129, Published: AUG 2004 Brazilian Journal of Medical and Biological Research, Vol 37, Iss 8, Pp 1119-1129 (2004) Brazilian Journal of Medical and Biological Research v.37 n.8 2004 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | Using a short-term bulk culture protocol designed for an intracellular-staining method based on a flow cytometry approach to the frequencies of cytokine-producing cells from tuberculosis and leprosy patients, we found distinct patterns of T cell subset expression. The method also reveals the profile of peak cytokine production and can provide simultaneous information about the phenotype of cytokine-producing cells, providing a reliable assay for monitoring the immunity of these patients. The immune response of Mycobacterium leprae and purified protein derivative (PPD) in vitro to a panel of mycobacteria-infected patients from an endemic area was assessed in primary mononuclear cell cultures. The kinetics and source of the cytokine pattern were measured at the single-cell level. IFN-gamma-, TNF-alpha-, IL-4- and IL-10-secreting T cells were intracytoplasmic evaluated in an attempt to identify M. leprae- and PPD-specific cells directly from the peripheral blood. The analysis by this approach indicated that TNF-alpha was the first (8 h) to be produced, followed by IFN-gamma (16 h), IL-10 (20 h) and IL-4 (24 h), and double-staining experiments confirmed that CD4+ were a greater source of TNF-alpha than of CD8+ T cells (P < 0.05). Both T cell subsets secreted similar amounts of IFN-gamma. We conclude that the protocol permits rapid evaluation of cytokine production by different T cell populations. The method can also be used to define immune status in non-infected and contact individuals. |
| Databáze: | OpenAIRE |
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