Expression of properdin in human monocytes
| Autor: | Johannes Möst, Christine Claus, H. W. Löms Ziegler-Heitbrock, Manfred P. Dierich, Hartwig P. Huemer, M Strobel, Wilhelm J. Schwaeble, K. B. M. Reid |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Lipopolysaccharides Lipopolysaccharide medicine.medical_treatment Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Biology urologic and male genital diseases Biochemistry Monocytes Cell Line Interferon-gamma chemistry.chemical_compound Tumor Cells Cultured medicine Humans RNA Messenger Northern blot Base Sequence Properdin Tumor Necrosis Factor-alpha Macrophages Monocyte Blotting Northern Molecular biology Recombinant Proteins Complement system Cytokine medicine.anatomical_structure chemistry Cell culture Immunology Alternative complement pathway Cytokines Tetradecanoylphorbol Acetate Interleukin-1 |
| Zdroj: | European Journal of Biochemistry. 219:759-764 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1994.tb18555.x |
| Popis: | Properdin is the only known positive regulator of the alternative pathway of complement activation. Northern blot analysis of cell lines derived from fibroblasts, B-cells, hepatoma cells, and cells of the monocyte-macrophage lineage revealed properdin expression only in the myelomonocytic cell line HL-60, in the monoblastic cell line U-937 and in the monocytic line Mono Mac 6. Culture of Mono Mac 6 cells for 24 h with phorbol 12-myristate 13-acetate, bacterial lipopolysaccharide and the cytokines interleukin-1 beta and tumour necrosis factor-alpha enhanced mRNA abundance, with the strongest effect (tenfold) being observed with the lipopolysaccharide. In contrast, recombinant interferon-gamma consistently halved properdin mRNA abundance. The same pattern was found for the secretion of properdin as detected by ELISA of Mono Mac 6 supernatants. The suppressive effect of interferon-gamma on properdin mRNA abundance was also demonstrated for primary blood monocytes. The data suggest that the expression and secretion of this complement regulatory protein by monocytes is differentially regulated by cytokines and link the immune response with alternative pathway activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|