Dense genetic linkage maps of three populus species (Populus deltoides, P. nigra and P. trichocarpa) Based on AFLP and microsatellite markers
| Autor: | Ben H Liu, Marc Van Montagu, Jaqueline Gusmão, Veronique Storme, María Teresa Cervera, Jos Van Slycken, Bart Ivens, Vanessa Hostyn, Wout Boerjan |
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| Předmět: |
Genetic Markers
Heterozygote Genetic Linkage Biology Quantitative trait locus Genes Plant Genome Trees Sequence-tagged site Genetic linkage Botany Genetics Crosses Genetic Sequence Tagged Sites Models Statistical Polymorphism Genetic Models Genetic Bulked segregant analysis Chromosome Mapping DNA Genetic marker Microsatellite Amplified fragment length polymorphism Research Article Microsatellite Repeats |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| Popis: | Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 × P. nigra cv. Ghoy and P. deltoides cv. S9-2 × P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73%, and 369 markers distributed over 28 groups for P. nigra covering 61%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome. |
| Databáze: | OpenAIRE |
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