Induction of a Mitosis Delay and Cell Lysis by High-Level Secretion of Mouse α-Amylase from Saccharomyces cerevisiae
| Autor: | Bi-Dar Wang, Tsong-Teh Kuo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Lysis Maturation-Promoting Factor Cell Saccharomyces cerevisiae Maturation promoting factor Mitosis Cell Cycle Proteins Applied Microbiology and Biotechnology Mice Gene Expression Regulation Fungal Phosphoprotein Phosphatases medicine Animals Protein Phosphatase 2 DNA Fungal Cell Cycle Protein Cyclin-dependent kinase 1 Ecology biology Cell cycle Physiology and Biotechnology biology.organism_classification Molecular biology Recombinant Proteins Culture Media medicine.anatomical_structure Mesothelin biology.protein alpha-Amylases CDC28 Protein Kinase S cerevisiae Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 67:3693-3701 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.67.8.3693-3701.2001 |
| Popis: | Some foreign proteins are produced in yeast in a cell cycle-dependent manner, but the cause of the cell cycle dependency is unknown. In this study, we found that Saccharomyces cerevisiae cells secreting high levels of mouse α-amylase have elongated buds and are delayed in cell cycle completion in mitosis. The delayed cell mitosis suggests that critical events during exit from mitosis might be disturbed. We found that the activities of PP2A (protein phosphatase 2A) and MPF (maturation-promoting factor) were reduced in α-amylase-oversecreting cells and that these cells showed a reduced level of assembly checkpoint protein Cdc55, compared to the accumulation in wild-type cells. MPF inactivation is due to inhibitory phosphorylation on Cdc28, as a cdc28 mutant which lacks an inhibitory phosphorylation site on Cdc28 prevents MPF inactivation and prevents the defective bud morphology induced by overproduction of α-amylase. Our data also suggest that high levels of α-amylase may downregulate PPH22, leading to cell lysis. In conclusion, overproduction of heterologous α-amylase in S. cerevisiae results in a negative regulation of PP2A, which causes mitotic delay and leads to cell lysis. |
| Databáze: | OpenAIRE |
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