A newly developed chemically crosslinked dextran-poly(ethylene glycol) hydrogel for cartilage tissue engineering
Autor: | Clemens van Blitterswijk, Leonardus J. van der Aa, Theun van Veen, Jan Feijen, Zhiyuan Zhong, Christine Hiemstra, Jan de Boer, J.M. Jukes, Pieter J. Dijkstra |
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Přispěvatelé: | Faculty of Science and Technology, Biomaterials Science and Technology |
Rok vydání: | 2009 |
Předmět: |
Cell Survival
Biomedical Engineering Bioengineering Embryoid body Biochemistry Hydrogel Polyethylene Glycol Dimethacrylate Cell Line Polyethylene Glycols Biomaterials chemistry.chemical_compound Mice Chondrocytes Tissue engineering Materials Testing medicine Cartilaginous Tissue Animals IR-68606 METIS-273581 Embryonic Stem Cells Glycosaminoglycans Tissue Engineering Cartilage technology industry and agriculture Cell Differentiation Dextrans Chondrogenesis medicine.anatomical_structure Dextran Cross-Linking Reagents chemistry Self-healing hydrogels Biophysics Cattle Ethylene glycol Biomedical engineering |
Zdroj: | Tissue engineering. Part A, 16(2), 565-573. Mary Ann Liebert |
ISSN: | 1937-335X 1937-3341 |
Popis: | Cartilage tissue engineering, in which chondrogenic cells are combined with a scaffold, is a cell-based approach to regenerate damaged cartilage. Various scaffold materials have been investigated, among which are hydrogels. Previously, we have developed dextran-based hydrogels that form under physiological conditions via a Michael-type addition reaction. Hydrogels can be formed in situ by mixing a thiol-functionalized dextran with a tetra-acrylated star poly(ethylene glycol) solution. In this article we describe how the degradation time of dextran-poly(ethylene glycol) hydrogels can be varied from 3 to 7 weeks by changing the degree of substitution of thiol groups on dextran. The degradation times increased slightly after encapsulation of chondrocytes in the gels. The effect of the gelation reaction on cell viability and cartilage formation in the hydrogels was investigated. Chondrocytes or embryonic stem cells were mixed in the aqueous dextran solution, and we confirmed that the cells survived gelation. After a 3-week culturing period, chondrocytes and embryonic stem cell-derived embryoid bodies were still viable and both cell types produced cartilaginous tissue. Our data demonstrate the potential of dextran hydrogels for cartilage tissue engineering strategies. |
Databáze: | OpenAIRE |
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