High-performance liquid chromatographic method for the determination of a novel leukotriene D4 antagonist (MK-0571) in biological specimens
| Autor: | Anthony G. Zacchei, Renee A. Berna |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Chromatography Leukotriene D4 Ultraviolet Rays Antagonist Rats Inbred Strains General Chemistry High-performance liquid chromatography Macaca mulatta Acute toxicity Rats Matrix (chemical analysis) Biological specimen chemistry.chemical_compound chemistry Liver Quinolines Animals Centrifugation Female SRS-A Methanol Propionates Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. 563(2) |
| Popis: | A rapid, sensitive and selective liquid chromatographic procedure was developed to quantitate the levels of a novel leukotriene D4 antagonist, MK-0571 (I), in biological samples. The method involves the addition of an internal standard, an analogue of I, and methanol to the biological matrix. Following centrifugation the supernatant is chromatographed isocratically on a C18 reversed-phase column and the acids are detected with an ultraviolet detector. The sensitivity of the method is such that 50 ng of drug can be quantitated per aliquot of sample. Assays were linear over a 0.06-40.0 micrograms range and exhibited a recovery of 100.5 +/- 7.0% (mean +/- S.D.) over this range. This procedure was utilized to monitor plasma, liver and urinary levels of I in chronic and acute toxicity studies in several animal species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|