Reduction in the amplitude of shortening and Ca(2+) transient by phlorizin and quercetin-3-O-glucoside in ventricular myocytes from streptozotocin-induced diabetic rats
| Autor: | Murat Oz, Juma Alkaabi, Frank Christopher Howarth, M. A. Qureshi, N. N. Hamouda |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system diseases Physiology Phlorizin Sodium Heart Ventricles chemistry.chemical_element 030204 cardiovascular system & hematology Streptozocin Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucosides Internal medicine medicine Myocyte Animals Myocytes Cardiac Cells Cultured Calcium metabolism Flavonoids Endoplasmic reticulum Glucose transporter nutritional and metabolic diseases General Medicine Streptozotocin Rats Sarcoplasmic Reticulum 030104 developmental biology Endocrinology Phlorhizin chemistry Calcium Quercetin medicine.drug |
| Zdroj: | Physiological research. 65(2) |
| ISSN: | 1802-9973 |
| Popis: | Diabetes mellitus is the leading cause of cardiovascular morbidity and mortality. Phlorizin (PHLOR) and quercetin-3-O-glucoside (QUER-3-G) are two natural compounds reported to have antidiabetic properties by inhibiting sodium/glucose transporters. Their effects on ventricular myocyte shortening and intracellular Ca(2+) in streptozotocin (STZ)-induced diabetic rats were investigated. Video edge detection and fluorescence photometry were used to measure ventricular myocyte shortening and intracellular Ca(2+), respectively. Blood glucose in STZ rats was 4-fold higher (469.64+/-22.23 mg/dl, n=14) than in Controls (104.06+/-3.36 mg/dl, n=16). The amplitude of shortening was reduced by PHLOR in STZ (84.76+/-2.91 %, n=20) and Control (83.72+/-2.65 %, n=23) myocytes, and by QUER-3-G in STZ (79.12+/-2.28 %, n=20) and Control (76.69+/-1.92 %, n=30) myocytes. The amplitude of intracellular Ca(2+) was also reduced by PHLOR in STZ (82.37+/-3.16 %, n=16) and Control (73.94+/-5.22 %, n=21) myocytes, and by QUER-3-G in STZ (73.62+/-5.83 %, n=18) and Control (78.32+/-3.54 %, n=41) myocytes. Myofilament sensitivity to Ca(2+) was not significantly altered by PHLOR; however, it was reduced by QUER-3-G modestly in STZ myocytes and significantly in Controls. PHLOR and QUER-3-G did not significantly alter sarcoplasmic reticulum Ca(2+) in STZ or Control myocytes. Altered mechanisms of Ca(2+) transport partly underlie PHLOR and QUER-3-G negative inotropic effects in ventricular myocytes from STZ and Control rats. |
| Databáze: | OpenAIRE |
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